Anti-ILK Polyclonal Antibody for WB, IHC-P, ELISA - Q13418

Core Product Specifications and Parameters

Biological Background: ILK Function and Localization

• ILK (Integrin-Linked Kinase), also known as ILK-1, ILK-2, and p59ILK, is a ubiquitously expressed scaffold protein that serves as a central adaptor in integrin-mediated cell adhesion and signaling.
• It forms the IPP (ILK-PINCH-PARVIN) complex by recruiting LIMS1 (PINCH) and PARVA (α-parvin), directly linking integrin cytoplasmic tails to the actin cytoskeleton and facilitating focal adhesion assembly and cell spreading (PubMed:30367047, PubMed:20005845). Related references: PMID:30367047, PMID:20005845
• ILK modulates key downstream pathways, including AKT1 and GSK3β, influencing cell survival and proliferation; its kinase domain is considered inactive, with scaffolding functions predominating (PubMed:9736715). Related references: PMID:9736715
• Beyond focal adhesions, ILK localizes to lamellipodia, cell cortex, sarcomeres, centrosomes, and the nucleus, where it may act as a transcriptional regulator (PubMed:17420447). Related references: PMID:17420447
• Tissue expression is highest in cardiac and skeletal muscle, with moderate levels in pancreas and kidney, and lower levels in placenta, lung, and liver, reflecting its roles in muscle integrity and function.
• ILK is critical for mitotic spindle organization via AURKA-mediated phosphorylation of TACC3, ensuring proper chromosome segregation (PubMed:18283114). Related references: PMID:18283114
• The protein contains ankyrin repeats and a pseudokinase domain, undergoes acetylation and phosphorylation, and is catalogued under keywords such as ANK repeat, ATP-binding, and cell junction.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-260) targets the N-terminal ankyrin repeat domain of human ILK, a region highly conserved among human, mouse, and rat, consistent with the reported cross-reactivity; this domain is critical for protein-protein interactions.
• In Western blotting, a band at approximately 51 kDa is expected; consider that post-translational modifications or isoform expression may lead to minor shifts.
• For immunohistochemistry (IHC-P), optimal results may be obtained with antigen retrieval and careful titration; ILK is enriched at focal adhesions and lamellipodia, so punctate membrane-proximal and cytoplasmic staining patterns are anticipated.
• For ELISA, the antibody can be used for capture or detection of recombinant ILK protein; pairing with a different anti-ILK antibody may improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material sourcing for cell adhesion and cytoskeletal research. This anti-ILK polyclonal antibody is part of our comprehensive portfolio supporting integrin signaling and focal adhesion studies. Contact us to discuss custom requirements or bulk orders.