Anti-ILF2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q12905

Core Product Specifications and Parameters

Biological Background: ILF2 Function and Localization

• ILF2 is a chromatin-interacting protein that forms a stable heterodimer with ILF3 (NF90), also known as nuclear factor of activated T-cells 45 kDa (NF45). Related references: PMID:18458058, PMID:31212927
• Together with ILF3, it regulates mitotic progression and cytokinesis by controlling mRNA stability of mitotic genes, competing with STAU1/STAU2-mediated decay. Related references: PMID:32433969
• ILF2 is essential for the nuclear reshuttling of ILF3 isoforms, indicating a role in nuclear-cytoplasmic transport.
• It plays a role in innate immunity, including inhibition of Japanese encephalitis virus and enterovirus 71.
• In HIV-1 infection, ILF2 positively regulates virus production by stabilizing viral RNA with ILF3.
• Subcellular localization includes the nucleus, nucleolus, and cytoplasm, consistent with its multifunctional roles.
• Post-translational modifications include phosphorylation, methylation, and ubiquitin-like conjugation (isopeptide bond).
• Molecular weight is approximately 43 kDa (UniProt: 43062 Da).

Experimental Guidance and Technical Tips

• The immunogen (aa 200-300) targets an internal sequence, potentially recognizing multiple isoforms; validate in your sample system.
• A band around 43 kDa is expected in Western blot; positive controls may include cell lines with known ILF2 expression.
• For immunohistochemistry, nuclear and nucleolar staining is anticipated; optimize fixation and antigen retrieval conditions.
• This rabbit monoclonal antibody may provide reproducible performance across batches.
• Cross-reactivity with mouse and rat is indicated; verify suitability for your species of interest.

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