Anti-IL9 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P15248

Core Product Specifications and Parameters

Biological Background: IL9 Function and Localization

• Interleukin-9 (IL9), also known as cytokine P40 or T-cell growth factor P40, is a multifunctional cytokine belonging to the IL-7/IL-9 family. It is primarily secreted by T-helper 2 (Th2) lymphocytes, mast cells, and NKT cells.
• IL9 plays a critical role in the immune response against parasitic infections and influences intestinal epithelial permeability and adaptive immunity. Related references: PMID:29742432
• This cytokine promotes the differentiation of IL-17-producing helper T-cells (Th17) and stimulates the proliferation and differentiation of mast cells.
• IL9 signals through a heterodimeric receptor complex consisting of the IL9R subunit and the common gamma chain (IL2RG), leading to activation of JAK1 and JAK3 kinases.
• Downstream signaling involves STAT1, STAT3, and STAT5; STAT1 primarily mediates differentiation gene induction, while STAT3 and STAT5 protect cells from apoptosis.
• The protein is secreted and undergoes post-translational modifications, including glycosylation and formation of pyrrolidone carboxylic acid at the N-terminus.
• According to UniProt, the calculated molecular weight of the unmodified precursor is approximately 15.9 kDa, consistent with the observed band at ~16 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19-144 of human IL9, representing the mature protein after signal peptide cleavage. This design targets the biologically active secreted form.
• For Western blotting, a band corresponding to ~16 kDa is expected under reducing conditions. Due to glycosylation, higher molecular weight species may be observed; validate in the appropriate sample type.
• In immunohistochemistry (paraffin-embedded sections), ensure antigen retrieval conditions are optimized for the tissue and fixation method used. Consider including relevant positive and negative tissue controls.
• This polyclonal antibody is also suitable for ELISA applications; however, pairing with a monoclonal detection antibody may improve specificity in sandwich ELISA formats. The user should validate the assay in their own system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive, one-stop sourcing platform for IVD raw materials, providing researchers and diagnostic manufacturers with reliable antibodies, antigens, and reagents for immunoassay development. This anti-IL9 polyclonal antibody supports studies in cytokine biology and immune regulation. As part of our broader portfolio, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials—helping streamline your transition from concept to clinical application.