Anti-IL9 Rabbit Monoclonal Antibody for WB, FC, ELISA - P15248

Core Product Specifications and Parameters

Biological Background: IL-9 Function and Localization

• IL-9 is a multifunctional cytokine secreted primarily by T-helper 2 lymphocytes, mast cells, and NKT cells, playing critical roles in anti-parasite immune responses and regulation of intestinal epithelial permeability and adaptive immunity. Related references: PMID:29742432
• It also promotes the differentiation of IL-17-producing helper T-cells (TH17) and the proliferation and differentiation of mast cells.
• IL-9 signals through a receptor composed of IL9R and the common gamma chain IL2RG, leading to JAK1/JAK3 kinase activation and subsequent STAT1, STAT3, and STAT5 transcriptional programs. STAT1 mediates differentiation gene induction, while STAT3 and STAT5 provide anti-apoptotic signals.
• It is a secreted protein, acting in the extracellular space to mediate intercellular communication.
• The protein is a glycoprotein and contains a signal peptide; it is classified under keywords including cytokine, growth factor, and pyrrolidone carboxylic acid.
• No isoforms are reported; the canonical protein has a molecular weight of approximately 15.9 kDa (UniProt) and migrates at around 16 kDa in SDS-PAGE.
• Tissue specificity is not explicitly described in this record, but its secretion by immune cells suggests roles in lymphoid and mucosal tissues.

Experimental Guidance and Technical Tips

• For Western blot, consider using reducing conditions; the theoretical molecular weight is 15.9 kDa, but glycosylation may cause a shift up to ~16-20 kDa.
• In intracellular flow cytometry, optimize cell stimulation and fixation/permeabilization protocols to detect cytokine production in activated T cells or mast cells.
• The antibody is raised against a recombinant protein corresponding to the mature human IL-9 sequence (aa 19-144), so it should recognize the secreted form; validate specificity in biological samples.
• Since IL-9 is a secreted cytokine, for detection in cell supernatants, consider using ELISA with appropriate capture/detection antibody pairs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. This anti-IL9 monoclonal antibody is part of our cytokine and immune monitoring portfolio, supporting the development of assays for infectious disease and immune-mediated disorders. Our supply capabilities cover bulk orders, custom production, and rare target material sourcing to accelerate your project from concept to clinic.