Anti-IL8 Rabbit Polyclonal Antibody for WB and ELISA - P10145

Core Product Specifications and Parameters

Biological Background: IL-8 Function and Localization

• Interleukin-8 (IL-8), also known as CXCL8, is a C-X-C motif chemokine that acts as a major mediator of the inflammatory response by attracting neutrophils, basophils, and T-cells to sites of infection or injury. Related references: PMID:18692776 PMID:7636208
• IL-8 exerts its effects by binding to the G protein-coupled receptors CXCR1 and CXCR2, primarily found on neutrophils, monocytes, and endothelial cells, leading to both chemotaxis and activation. Related references: PMID:1840701 PMID:1891716 PMID:2145175 PMID:9623510
• Upon receptor activation, IL-8 triggers the release of G protein beta and gamma subunits from Galpha (GNAI2 in neutrophils), activating downstream signaling pathways including PI3K and MAPK. Related references: PMID:11971003 PMID:8662698
• IL-8 is a secreted protein with a molecular weight of approximately 11 kDa, containing conserved disulfide bonds essential for structural stability and subject to post-translational modifications such as citrullination.
• High-resolution three-dimensional structures have elucidated the dimeric architecture of IL-8 and provided insights into receptor-binding interfaces.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody corresponds to the N-terminal region (amino acids 1-99) of human IL-8, suggesting reactivity with both full-length and mature forms of the protein.
• For Western blotting, a band around 11 kDa is expected; however, dimer formation or glycosylation may yield additional bands. Validating under reducing/non-reducing conditions may help clarify band patterns.
• This polyclonal antibody can be used for ELISA detection of IL-8 in serum, plasma, or cell culture supernatants, but assay conditions (capture/detection pairing, sample preparation) should be optimized in-house.
• Cross-reactivity has been confirmed for human IL-8; reactivity with other species or related chemokines (e.g., CXCL1, CXCL2) should be experimentally determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This anti-IL8 polyclonal antibody is part of our comprehensive portfolio for inflammation and immunology research. From validated antibody pairs and bulk monoclonal/polyclonal reagents to ancillary raw materials and rare-target sourcing, we support your assay development from concept to clinic.