Anti-IL7 Rabbit Polyclonal Antibody for WB, ELISA - P13232

Core Product Specifications and Parameters

Biological Background: IL-7 Function and Localization

• Interleukin-7 (IL-7) is a hematopoietic cytokine critical for the development, expansion, and survival of naive and memory T-cells and B-cells, thereby maintaining lymphoid homeostasis. Related references: PMID:25870237, PMID:7527823
• IL-7 signals through a heterodimeric receptor composed of IL7RA and CSF2RG, leading to activation of JAK1 or JAK3 kinases. Related references: PMID:8128231
• Downstream signaling includes the PI3K/Akt/mTOR and JAK-STAT5 pathways, which drive cell proliferation, survival, and differentiation. Related references: PMID:18523275, PMID:20974963
• IL-7 is a secreted glycoprotein with a predicted molecular weight of about 20 kDa, featuring disulfide bonds and glycosylation important for its stability and function.
• The human IL7 gene generates multiple isoforms via alternative splicing, and genetic variants are linked to immunodeficiencies and autoimmune conditions.
• Keywords for IL-7 include: 3D-structure, alternative splicing, cytokine, disease variant, disulfide bond, glycoprotein, growth factor, secreted, signal.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 26–177 of human IL-7, which likely generates antibodies recognizing multiple epitopes. Given the polyclonal nature, batch-to-batch consistency should be verified for critical assays.
• For Western blot (WB), expect a band near 20 kDa under reducing conditions. Use appropriate tissue or cell lysates known to express IL-7, such as activated T-cells or thymic stroma. Validate antibody specificity using a blocking peptide or recombinant IL-7 if available.
• In ELISA applications, consider using direct or sandwich formats with recombinant human IL-7 as a standard. The antibody's cross-reactivity with mouse and rat IL-7 allows for comparative studies, but quantitative assays should be validated with species-matched standards.
• As IL-7 is a secreted factor, cell culture supernatants or serum samples may contain low endogenous levels; enrichment or stimulation (e.g., with PMA/ionomycin for T-cells) might be necessary for detection.
• Always include positive and negative controls, such as lysates from IL-7-overexpressing cells or IL-7 knockout models, to confirm band identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a single point of access to a broad repertoire of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For investigations centered on IL-7 and lymphocyte development, our sourcing network can supply rare-target reagents and scale-up support, smoothing the transition from early-stage research to clinical application. Connect with our team to accelerate your assay development pipeline with dependable, high-quality materials.