Anti-IL6ST Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P40189

Core Product Specifications and Parameters

Biological Background: IL6ST Function and Localization

• IL6ST, also known as gp130, CD130, or interleukin-6 receptor subunit beta, is a ubiquitously expressed transmembrane protein that serves as the common signal-transducing subunit for the IL-6 cytokine family, including IL-6, LIF, OSM, CNTF, IL-11, CTF1, and others. Related references: PMID:11285233, PMID:11294841, PMID:1542794

• Upon cytokine binding, IL6ST undergoes homodimerization (for IL-6 or IL-11) or heterodimerization with LIFR or OSMR (for other family members), leading to activation of JAK tyrosine kinases and downstream STAT signaling, as well as YAP and NOTCH pathways. Related references: PMID:19915009, PMID:2261637, PMID:23294003, PMID:39532904, PMID:8999038

• IL6ST also binds the soluble IL-6/sIL-6R complex (hyper-IL-6), inhibiting IL-6 trans-signaling and the acute phase response, and it can block IL-11 cluster signaling through IL-11R. Related references: PMID:11121117, PMID:21990364, PMID:30279168

• The protein is located on the cell membrane and can also be secreted; it is found in all tissues and cell lines tested and is present in blood serum. Related references: PMID:2261637, PMID:24629561

• IL6ST is a glycoprotein with a calculated molecular weight of ~103.5 kDa (observed at ~104 kDa), featuring immunoglobulin-like domains, disulfide bonds, and multiple phosphorylation sites; it plays roles in immune response, hematopoiesis, bone metabolism, pain control, and embryonic development.

• The gene for IL6ST (Gene ID 3572 in human) gives rise to multiple isoforms via alternative splicing; mutations are associated with several diseases including hyper-IgE recurrent infection syndrome and inflammatory bowel disease.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 678–857) lies within the intracellular region, so the antibody may recognize both full-length and truncated forms; verify specificity in your experimental system.
• For Western blotting, expect a band at ~104 kDa; additional bands may represent splice isoforms or degradation products—optimize sample preparation and loading controls.
• This polyclonal antibody has been reported to work in IHC-P and ELISA; for IHC-P, consider antigen retrieval methods and test recommended dilutions starting from 1:50 to 1:200.
• Cross-reactivity with mouse and rat is predicted based on sequence homology; validate in the relevant sample system if working with these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a full spectrum of IVD raw materials, from validated antibody pairs to optimized monoclonal/polyclonal antibodies and ancillary reagents. Our IL6ST Rabbit pAb supports in‑depth investigation of cytokine signaling, immune regulation, and inflammatory disease pathways. Reach out to explore bulk sourcing, rare‑target material procurement, and custom solutions tailored to your assay development pipeline.