Anti-IL5RA Rabbit Polyclonal Antibody for WB, ELISA - Q01344

Core Product Specifications and Parameters

Biological Background: Interleukin-5 Receptor Subunit Alpha Function and Localization

• IL5RA functions as the ligand-binding alpha subunit of the heterodimeric IL-5 receptor, pairing with CSF2RB to bind interleukin-5. Related references: PMID:1495999 PMID:22528658
• It plays a critical role in eosinophil biology, mediating survival, differentiation, and chemotaxis. Related references: PMID:9378992
• In the absence of ligand, IL5RA constitutively associates with JAK2; receptor activation triggers the JAK-STAT signaling cascade. Related references: PMID:9516124
• The receptor is a transmembrane glycoprotein with a calculated molecular weight of approximately 48 kDa (consistent with UniProt annotation of 47.7 kDa).
• Expression is largely restricted to eosinophils and basophils, aligning with its role in allergic inflammation and parasitic responses.
• IL5RA is subject to alternative splicing and contains disulfide bonds, as annotated in UniProt.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 20–335); consider that the antibody likely recognizes the native receptor ectodomain.
• For Western blot, a predominant band around 48 kDa is expected; additional bands may arise from glycosylation or alternatively spliced isoforms.
• In ELISA, the antibody may be evaluated as a capture or detection reagent; cross-reactivity with mouse and rat suggests utility in preclinical models, but validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare-target procurement. Our IL5RA rabbit polyclonal antibody supports eosinophil-related research and assay development, and we stand ready to deliver comprehensive supply solutions tailored to your diagnostic or research program.