Anti-IL4I1 Polyclonal Antibody for WB, ELISA - Q96RQ9

Core Product Specifications and Parameters

Biological Background: IL4I1 Function and Localization

• Protein identity and isoforms: IL4I1 (L-amino-acid oxidase, also known as interleukin-4-induced protein 1 or FIG1) is a secreted flavoprotein belonging to the L-amino acid oxidase family. Encoded by the IL4I1 gene, it generates multiple isoforms through alternative splicing and alternative promoter usage.
• Core enzymatic function: IL4I1 catalyzes the oxidative deamination of L-amino acids, with preference for aromatic residues: phenylalanine, tyrosine, and tryptophan are converted to phenylpyruvic acid, hydroxyphenylpyruvic acid, and indole-3-pyruvic acid, respectively. It also displays weak L-arginine oxidase activity. Related references: PMID:17356132, PMID:32818467, PMID:32866000, PMID:26673964
• Immunoregulatory role via AHR activation: By degrading tryptophan, IL4I1 generates indole metabolites (indole-3-pyruvic acid, indole-3-acetic acid, indole-3-aldehyde) and kynurenic acid that act as ligands for the aryl hydrocarbon receptor (AHR). AHR activation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Related references: PMID:32818467, PMID:32866000
• Modulation of immune cell function: IL4I1 inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4+ T cells into FOXP3+ regulatory T cells, and regulates B-cell development. It also drives M2 macrophage polarization by inhibiting T-cell activation. Related references: PMID:17356132, PMID:25446972, PMID:25778793
• Antibacterial activity: The enzyme exerts antibacterial effects against both Gram-negative and Gram-positive bacteria through the production of ammonium and hydrogen peroxide. Related references: PMID:23355881
• Subcellular localization: IL4I1 is secreted and additionally found in lysosomes, cytoplasmic vesicles, and secretory vesicles including acrosomes.
• Tissue and disease expression: Highest expression occurs in immune tissues (lymph nodes, spleen), with protein detected in germinal center macrophages, inflammatory myeloid cells, antigen-presenting cells, and spermatozoa. It is highly expressed in primary mediastinal large B-cell lymphoma and other B-cell lymphomas, as well as in tumor-associated macrophages.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 22–220) of mature IL4I1, which may facilitate detection of secreted and processed forms.
• For Western blot, consider using reducing conditions and probing cell lysates or conditioned media from immune cells such as macrophages or dendritic cells, given the secreted and lysosomal nature of IL4I1.
• In ELISA applications, this polyclonal antibody can serve as a capture or detection reagent after appropriate pairing and validation with a matched antibody.
• The reported cross-reactivity with mouse and rat suggests utility in preclinical models; confirm specificity via appropriate controls in those species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by providing a single-source supply chain for critical raw materials. For researchers targeting IL4I1 in immunoregulation and cancer biology, we offer not only this polyclonal antibody but also validated monoclonal and recombinant alternatives, matched detection pairs, and bulk ancillary reagents to accelerate assay development. Let our team assist with sourcing rare-target reagents and customized production to bridge your project from concept to clinic.