Anti-IL4 Rabbit Monoclonal Antibody for WB, ELISA - P07750

Core Product Specifications and Parameters

Biological Background: IL-4 Function and Localization

• Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1 (BSF-1), is a pleiotropic cytokine encoded by the Il4 gene.
• IL-4 is secreted primarily by mast cells, T-cells, eosinophils, and basophils and plays a key role in regulating antibody production, hematopoiesis, inflammation, and effector T-cell responses (PubMed:3083412). It induces expression of class II MHC molecules on resting B-cells and enhances secretion and cell surface expression of IgE and IgG1 (PubMed:3498301). Related references: PMID:3083412, PMID:3498301
• IL-4 positively regulates IL31RA expression in macrophages and stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and inducing RUFY4 (PubMed:26416964). Related references: PMID:26416964
• Upon binding to its receptor, IL-4 triggers dimerization of IL4R with either IL2RG (type 1 complex, hematopoietic cells) or IL13RA1 (type 2 complex, nonhematopoietic cells), leading to JAK3/JAK1 phosphorylation and STAT6 activation (PubMed:25847241, PubMed:8624821). Related references: PMID:25847241, PMID:8624821
• IL-4 plays a critical role in higher brain functions such as memory and learning (PubMed:25772794, PubMed:28202615). Related references: PMID:25772794, PMID:28202615
• The protein is secreted and contains glycosylation sites and disulfide bonds that are important for its structure and function.
• It acts as a growth factor and is involved in B-cell activation, as indicated by its keyword annotations.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment covering amino acids 21–140 of mouse IL-4. This region corresponds to the mature secreted form; it is advisable to confirm reactivity with the processed protein in the relevant sample system.
• For WB, the predicted molecular weight is ~16 kDa; consider using a low molecular weight marker and appropriate transfer conditions. Due to secretion, cell culture supernatants or concentrated samples may be required.
• For ELISA, pairing with a capture antibody against a different epitope may improve sensitivity. Optimize coating and detection conditions using recombinant mouse IL-4 as standard.
• As the antibody cross-reacts with mouse IL-4, validate specificity in murine samples and be aware of potential interference from soluble cytokine in complex matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies for cytokine targets such as IL-4. Alongside this anti-mouse IL-4 antibody, we offer matched antibody pairs, ancillary reagents, and bulk ordering options to streamline your assay development. From initial screening to scale-up, our sourcing service connects you with the specialized components needed for immunology and inflammation research.