Anti-IL36G Polyclonal Antibody for WB, ELISA - Q9NZH8

Core Product Specifications and Parameters

Biological Background: IL36G Function and Localization

• IL36G, also known as IL-1F9 or IL-1H1, is a member of the interleukin-1 (IL-1) cytokine family, structurally related to IL-1 beta and implicated in inflammatory signaling at epithelial surfaces.
• It binds to the IL1RL2/IL-36R receptor and shares the coreceptor IL1RAP with the IL-1 system, activating NF-kappa-B and MAPK pathways to drive pro-inflammatory gene expression.
• The protein is found in the cytoplasm and secreted, acting on keratinocytes, dendritic cells, and indirectly on T cells to promote immune cell infiltration and proliferation.
• In skin, IL36G is highly expressed in keratinocytes and upregulated in lesional psoriasis, where it induces chemokines such as CCL3, CCL4, CXCL8, and stimulates its own expression and that of TNF and S100A7/psoriasin.
• Beyond skin, it is expressed in lung, stomach, and esophageal epithelia, and contributes to neutrophilic airway inflammation by activating mitogen-activated protein kinases and NF-kappa-B in lung fibroblasts, inducing IL-8 and CXCL3.
• IL36G participates in innate immune responses to fungal pathogens like Aspergillus fumigatus, indicating a role in epithelial antimicrobial defense.
• The gene undergoes alternative splicing, and the protein features a 3D structure with a predicted molecular weight close to 19 kDa, consistent with the product specification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-169 of human IL36G, covering the mature secreted form; consider using the antibody in Western blotting under reducing conditions to detect endogenous protein near 19 kDa.
• For ELISA applications, optimize coating and detection conditions; pairing with a monoclonal or another polyclonal antibody against IL36G may improve sensitivity in sandwich-type assays.
• Given cross-reactivity with mouse and rat, validate the antibody in tissue lysates from these species, keeping in mind potential variations in expression levels between normal and inflamed samples.
• As a polyclonal antibody, it may recognize linear epitopes; careful titration and blocking are recommended to minimize background in immunohistochemical or cell-based applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material sourcing, providing this IL36G polyclonal antibody alongside a full portfolio of reagents for cytokine and inflammatory disease research. We supply validated antibody pairs, bulk ancillary reagents, and rare target sourcing solutions to accelerate your diagnostic assay development from concept to clinic. Let us streamline your procurement for reliable, high-quality materials in skin inflammation and innate immunity studies.