Anti-IL33 Rabbit Polyclonal Antibody for WB, ELISA - Q8BVZ5

Core Product Specifications and Parameters

Biological Background: IL-33 Function and Localization

• IL-33 (also known as Il-33, Il1f11, NF-HEV, or 9230117N10Rik) is a dual-function protein of the IL-1 cytokine family. The full-length precursor can be processed into mature cytokine forms.
• As a cytokine, IL-33 binds to the IL1RL1/ST2 receptor and activates NF-κB and MAPK signaling pathways, driving Th2 cell maturation and type 2 cytokine secretion. Related references: PMID:29045903
• IL-33 functions as an alarmin, released upon tissue damage to act as a chemoattractant for Th2 cells and to activate mast cells, basophils, eosinophils, and natural killer cells (By similarity).
• IL-33 induces rapid UCP2‑dependent mitochondrial rewiring in macrophages, lowering reactive oxygen species and sustaining itaconate production to promote GATA3‑dependent differentiation of alternatively activated macrophages. Related references: PMID:34644537
• In quiescent endothelial cells, uncleaved IL-33 resides in the nucleus, associating with chromatin and acting as a transcriptional repressor by sequestering NF-κB/RELA (By similarity).
• Subcellularly, IL-33 is detected in the nucleus, chromosomes, cytoplasm, cytoplasmic vesicles, and can be secreted, reflecting its multifaceted functions.
• The mature protein has a calculated molecular weight of approximately 30 kDa, consistent with the full‑length murine IL-33.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 109‑266 of mouse IL‑33) covers a region within the cytokine domain; the antibody may recognize both full‑length and proteolytically processed forms. Consider this when interpreting bands differing from the predicted ~30 kDa in WB.
• For Western blotting, a band near 30 kDa is expected in mouse and rat tissue or cell lysates. Validate in the relevant sample system and compare with known expression patterns.
• IL‑33 can be detected by ELISA in various biological fluids; for sandwich ELISA, pair this pAb with a validated capture antibody (monoclonal) and perform titration to optimize signal.
• Cross‑reactivity with rat is reported; for other species or specific applications, confirm by pilot experiments.
• Shipping on ice bags ensures antibody stability; upon receipt, aliquot and store at recommended temperatures to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers and researchers with validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare‑target procurement. The IL‑33 Rabbit pAb supports immunological investigations into Th2‑mediated responses, alarmin functions, and nuclear cytokine biology. Contact our team to discuss custom antibody development or bulk supply needs to accelerate your assay development workflow.