Anti-IL33 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - O95760

Core Product Specifications and Parameters

Biological Background: Interleukin-33 Function and Localization

• Interleukin-33 (IL-33), also known as IL-1F11 and NF-HEV, is a dual-function protein encoded by the IL33 gene (C9orf26, IL1F11, NFHEV). It exists as both a secreted cytokine and a nuclear factor, with alternative splicing generating multiple isoforms.
• As a cytokine, IL-33 binds to the IL1RL1/ST2 receptor, activating NF-kappa-B and MAPK pathways. It promotes Th2 cell maturation and secretion of type 2 cytokines, and activates mast cells, basophils, eosinophils, and natural killer cells. Related references: PMID:16286016 PMID:19841166 PMID:17853410 PMID:18836528
• IL-33 enhances alternative macrophage polarization and acts as a chemoattractant for Th2 cells, functioning as an 'alarmin' released upon tissue injury to amplify immune responses. Related references: PMID:19841166 PMID:17853410 PMID:18836528
• The uncleaved nuclear form of IL-33 is highly expressed in quiescent endothelial cells, where it acts as a chromatin-associated transcriptional repressor by sequestering NF-kappaB/RELA, reducing pro-inflammatory gene expression. This form is rapidly lost upon angiogenic or pro-inflammatory activation. Related references: PMID:21734074 PMID:18787100
• IL-33 induces rapid UCP2-dependent mitochondrial rewiring in macrophages, attenuating reactive oxygen species and preserving Krebs cycle integrity for persistent itaconate production, which supports the differentiation of inflammation-resolving alternatively activated macrophages (by similarity).
• Subcellular localization varies between the nuclear form (nucleus, chromosome) and the secreted form (cytoplasm, cytoplasmic vesicles, secretion), reflecting its dual functionality.
• Tissue specificity: Expressed at high levels in high endothelial venules of tonsils, Peyer's patches, and mesenteric lymph nodes; almost undetectable in placenta.
• Keywords: 3D-structure, alternative splicing, chromosome, cytokine, cytoplasm, cytoplasmic vesicle, direct protein sequencing, nucleus, proteomics identification, reference proteome, secreted, transcription.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 109-270 of human IL33 (NP_001300974.1), which covers the mature cytokine domain. For WB, consider using positive controls such as stimulated endothelial cells or recombinant IL-33, and note that full-length and cleaved forms may both be detected at masses around 31 kDa or lower.
• For IF/ICC and IHC-P, validate nuclear and cytoplasmic staining patterns in relevant tissue sections or cell lines expressing IL-33; perform appropriate controls to confirm specificity.
• ELISA applications can be developed using this antibody as a capture or detection reagent; titrate both antibody and antigen to optimize signal-to-noise ratio.
• Given the dual nuclear and secreted functions, select sample preparation protocols that preserve both compartments if investigating total IL-33 expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized recombinant antibodies, bulk ancillary reagents, and rare target sourcing. This anti-IL33 rabbit monoclonal antibody exemplifies our ability to supply critical tools for immune response and inflammatory disease research, from early-stage biomarker discovery to clinical assay development. Whatever your project demands—custom formulations, technical consultation, or consistent lot-to-lot performance—CamelBio is equipped to support your path from concept to clinic.