Anti-IL31RA Polyclonal Antibody for WB, ELISA - Q8NI17

Core Product Specifications and Parameters

Biological Background: IL31RA Function and Localization

• IL31RA functions as the signal-transducing subunit of the IL31 receptor complex, heterodimerizing with OSMR to bind IL31 and mediate downstream signaling crucial for skin immunity. Related references: PMID:15184896, PMID:15194700, PMID:15627637, PMID:11877449
• Upon ligand binding, the receptor activates JAK1/JAK2/TYK2 kinases, which phosphorylate tyrosine residues creating docking sites for STAT1, STAT3, and STAT5B, and triggers the MAPK pathway via SHC1.
• Subcellularly, IL31RA localizes to the cell membrane, presynaptic cell membrane, and axonal projections, consistent with roles in immune and neuronal signaling.
• The protein is expressed in immune cells (CD14+ and CD56+ blood cells, macrophages), keratinocytes, and a subset of dorsal root ganglia neurons, with low levels in various tissues. Isoform 6 shows elevated expression in lesional skin of atopic dermatitis patients.
• IL31RA belongs to the type I cytokine receptor family and contains fibronectin type III domains; its gene undergoes alternative splicing to produce multiple isoforms.
• Keywords highlight involvement in immunity, amyloidosis, disease variants, and post-translational modifications like glycosylation.
• The receptor mediates IL31-induced itch, likely through TRPA1 and TRPV1 channels, and positively regulates myeloid progenitor cell survival and cycling in bone marrow.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 20-321), conferring reactivity against both human and mouse orthologs (cross-reactivity confirmed for mouse).
• For Western blot, the predicted molecular weight is 83 kDa, but glycosylation and isoforms may shift bands. Validate with positive controls such as IL31RA-expressing cell lines or skin tissues.
• In ELISA applications, consider using the antibody for capture or detection of recombinant or native IL31RA. Titration and optimization are recommended.
• Due to limited expression in some tissues, enrichment strategies like immunoprecipitation may improve detection sensitivity.
• The antibody ships on ice bags; aliquot upon receipt to avoid freeze-thaw cycles and store at recommended conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a seamless supply chain for high-quality IVD raw materials. This anti-IL31RA antibody supports studies on inflammatory skin diseases and pruritus signaling. From validated antibody pairs to bulk ancillary reagents, we provide one-stop access to accelerate your assay development from concept to clinic.