Anti-IL31 Rabbit Polyclonal Antibody for WB, ELISA - Q6EBC2

Core Product Specifications and Parameters

Biological Background: IL-31 Function and Localization

• IL-31 is a cytokine that signals through a heterodimeric receptor complex composed of OSMR and IL31RA (PubMed:15184896, PubMed:15627637).
  Related references: PMID:15184896 PMID:15627637
• The interaction of IL-31 with IL31RA induces heterodimerization with OSMR, leading to activation of JAK1, JAK2, and to a lesser extent TYK2 kinases (PubMed:15627637).
  Related references: PMID:15627637
• Downstream signaling involves phosphorylation of STAT1, STAT3, and STAT5B, and activation of the MAPK pathway (ERK1/ERK2) via SHC1 adaptor recruitment (PubMed:15184896, PubMed:15627637).
  Related references: PMID:15184896 PMID:15627637
• IL-31 is particularly important in skin immunity and has been implicated in inflammatory skin conditions (PubMed:15184896).
  Related references: PMID:15184896
• It enhances myeloid progenitor cell survival in vitro and induces RETNLA and serum amyloid A protein expression in macrophages (By similarity).
• IL-31 is a secreted glycoprotein, consistent with its role as a circulating cytokine.
• Tissue expression is detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine, and trachea.
• The protein has a calculated molecular weight of approximately 18.2 kDa (UniProt), matching the observed 18 kDa product specification.

Experimental Guidance and Technical Tips

• For Western blot analysis, validate antibody specificity in your sample system by including appropriate controls, such as IL-31-overexpressing or knockout lysates, to confirm band identity.
• Since IL-31 is a secreted cytokine, consider enriching cell culture supernatants or using immunoprecipitation to improve detection sensitivity when analyzing endogenous levels.
• When developing ELISA-based assays, this polyclonal antibody may be explored as a capture or detection reagent; however, pairing with a matched monoclonal antibody is recommended for specific sandwich immunoassays.
• Given the reported cross-reactivity with mouse and rat IL-31, verify species reactivity using relevant positive controls, as epitope conservation may influence binding affinity and background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing bridge for IVD raw materials. In addition to this anti-IL31 polyclonal antibody, we offer a full portfolio of validated antibody pairs, optimized recombinant proteins, and ancillary reagents to accelerate your cytokine and skin immunity research. Our one-stop platform enables diagnostic manufacturers, labs, and research institutes to efficiently secure rare target raw materials and transition from concept to clinic.