Anti-IL3 Rabbit Polyclonal Antibody for WB, ELISA - P08700

Core Product Specifications and Parameters

Biological Background: IL-3 Function and Localization

• Interleukin-3 (IL-3), also known as mast cell growth factor (MCGF), multipotential colony-stimulating factor (MULTI-CSF), and P-cell-stimulating factor, is a cytokine secreted predominantly by activated T-lymphocytes, mast cells, and osteoblastic cells. It controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells.
  Related references:
  PMID:2556442

• IL-3 stimulates mature basophils, eosinophils, and monocytes to become functionally activated, contributing to immune effector responses.
  Related references:
  PMID:10779277
  PMID:32889153

• Beyond hematopoiesis, IL-3 plays a role in neural cell proliferation and survival, indicating broader functions in the nervous system.
  Related references:
  PMID:23226269

• IL-3 participates in bone homeostasis by inhibiting osteoclast differentiation via preventing NF-kappa-B nuclear translocation and activation.
  Related references:
  PMID:12816992

• Mechanistically, IL-3 signals through a heterodimeric receptor composed of the IL3RA subunit and the signal transducing subunit IL3RB. Receptor engagement activates JAK2 kinase and the STAT5 transcriptional program.
  Related references:
  PMID:29374162

• Under oxidative stress, IL-3 can promote cell survival in non-hematopoietic systems by activating PI3K/AKT and ERK pathways.
  Related references:
  PMID:27862234

• IL-3 is a secreted glycoprotein with disulfide bonds, classified as a hematopoietic growth factor. Its expression is primarily in activated T-cells, mast cells, and natural killer cells, consistent with its immune regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–152 of human IL-3, which represents the mature secreted protein after cleavage of the signal peptide. This design may ensure recognition of the physiologically relevant processed form.
• For Western blotting, a band around 17 kDa is expected under reducing conditions; however, post-translational modifications such as glycosylation may cause a higher apparent molecular weight. Validate in the specific sample type and optimize antibody dilution.
• For ELISA, this antibody can be utilized to detect IL-3 levels in cell culture supernatants or other biological fluids. Pair with a suitable matched antibody set if developing a sandwich assay; cross-reactivity with mouse IL-3 enables testing in murine model systems.
• As with any polyclonal antibody, confirm specificity in your application using appropriate negative controls and recombinant protein standards. Consider potential batch-to-batch variability and validate each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing bridge, supplying manufacturers and research institutes with validated antibodies, including anti-IL3 polyclonal antibodies like this one, alongside antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. We streamline procurement from concept to clinic, supporting your cytokine research and immunoassay development with reliable, fully traceable materials.