Anti-IL26 Rabbit Polyclonal Antibody for WB, ELISA - Q9NPH9

Core Product Specifications and Parameters

Biological Background: IL-26 Function and Localization

• IL-26 (Interleukin-26), also designated AK155, is a secreted cytokine of the IL-10 family.
• It contributes to mucosal immunity and exhibits pro-inflammatory activity, notably in the context of inflammatory bowel disease (IBD).
• IL-26 triggers intracellular signaling through activation of STAT1, STAT3, MAPK1/3 (ERK1/2), JUN, and AKT pathways.
• It induces expression of SOCS3, TNF, IL-8, and surface ICAM1, and stimulates secretion of IL-8 and IL-10.
• IL-26 reduces proliferation of intestinal epithelial cells, and its function is antagonized by heparin.
• The protein is primarily expressed in HVS-transformed T-cells, colonic T-cells (including Th17 cells), and is elevated in serum of Crohn’s disease patients.
• IL-26 contains a signal peptide and is secreted as a mature ~20 kDa protein (UniProt: 19843 Da).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 22–100 (N-terminal region) of human IL-26. Validation of specificity using recombinant protein or relevant cell lysates is recommended.
• For Western blotting, the predicted band size is ~20 kDa; possible post‑translational modifications may alter migration. Use appropriate molecular weight markers.
• Reported cross‑reactivity with mouse IL-26 makes this antibody a candidate for mouse studies; however, consistency should be verified in your model system.
• In ELISA applications, optimize coating conditions and include controls to assess potential cross‑reactivity with other IL-10 family cytokines.
• As IL-26 is secreted, activated T‑cell culture supernatants or serum samples (e.g., from IBD models) are suitable for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, providing reliable access to validated polyclonal antibodies, like this anti-IL26 pAb, for cytokine research and inflammatory disease assay development. We supply bulk quantities, technical consultation, and supporting reagents—empowering diagnostic manufacturers and labs to progress from discovery to clinical application efficiently.