Anti-IL24 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13007

Core Product Specifications and Parameters

Biological Background: IL24 Function and Localization

• IL24 (Interleukin-24), also known as MDA-7 or ST16, is a secreted glycoprotein belonging to the IL-10 cytokine family.
• It is a multifunctional cytokine primarily produced by T-cells, with roles in immune regulation, tissue homeostasis, host defense, and oncogenesis. Related references: PMID:25168428 PMID:27687232
• Exhibits antiviral activity by inducing type I interferon responses during influenza infection. Related references: PMID:27687232
• Signals through heterodimeric receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1, activating JAK1-STAT3 and MAPK pathways, leading to secretion of pro-inflammatory mediators like IL8 and MMP1. Related references: PMID:11706020 PMID:30111632 PMID:25168428
• Intracellularly, IL24 maintains endoplasmic reticulum homeostasis by restricting eIF2alpha-CHOP stress signaling, and monitors proteasome function via PKR/EIF2AK2 activation.
• Up-regulated during terminal differentiation of melanoma cells, reflecting its tumor-suppressive and pro-differentiation roles.
• Undergoes alternative splicing and post-translational modifications including glycosylation and disulfide bond formation, as indicated by curated keywords.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 52-140 of human IL24, covering the predicted mature cytokine region. This design may facilitate detection of both secreted and intracellular protein pools.
• For Western blot (WB), a band near 24 kDa is expected under denaturing and reducing conditions; validation with positive controls (e.g., stimulated immune cells or recombinant protein) is recommended.
• In immunohistochemistry (IHC-P), antigen retrieval methods and appropriate tissue controls (e.g., melanoma or immune cell-rich tissues) should be optimized to confirm specificity.
• The antibody cross-reacts with mouse and rat IL24; however, sequence alignment of the immunogen region is advised to assess epitope conservation.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a matched antibody pair may improve assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers, laboratories, and research teams with high-quality IVD raw materials and comprehensive technical support from concept to clinic. For researchers studying IL24-mediated immune responses, antiviral mechanisms, and oncogenesis, CamelBio offers validated polyclonal antibodies, recombinant proteins, and custom antibody development services to accelerate assay development. As your sourcing partner, we ensure access to reliable reagents, bulk ancillary supplies, and rare-target raw materials for seamless IVD workflow integration.