Anti-IL23R Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q5VWK5

Core Product Specifications and Parameters

Biological Background: IL23R Function and Localization

• Interleukin-23 receptor (IL23R) associates with IL12RB1 to form a functional receptor complex that specifically binds IL23.
• Upon ligand binding, IL23R mediates signaling through the Jak-Stat cascade, triggering stimulation of T-cells, NK cells, and possibly certain macrophage/myeloid cells.
• IL23 plays a dual role in innate and adaptive immunity, contributing to acute responses against infection in peripheral tissues.
• Dysregulation of IL23/IL23R signaling is implicated in autoimmune inflammatory diseases and may promote tumorigenesis.
• IL23R is a cell membrane receptor and a single-pass transmembrane protein, possessing a signal peptide, an extracellular ligand-binding domain, and a cytoplasmic signaling domain.
• Expression has been detected in monocytes, Th1, Th0, NK, and dendritic cells; an alternatively spliced isoform (isoform 1) is specifically expressed in NK cells.
• The receptor undergoes glycosylation and phosphorylation, modifications that are critical for its cell-surface localization and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 510–629 within the cytoplasmic domain of human IL23R, thus the antibody is expected to recognize the total receptor pool regardless of potential extracellular domain shedding.
• For Western blot, a band near the predicted 72 kDa molecular weight should be observed; lysates from stimulated immune cells (e.g., PBMCs or cell lines expressing IL23R) may be suitable.
• For IHC-P, consider testing in tissues with known IL23R expression (e.g., lymphoid organs, inflamed tissues) and optimize antigen retrieval conditions.
• In ELISA, this antibody can be explored as a capture or detection reagent; pairing with another clone or a polyclonal antibody is advised for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research labs worldwide. This IL23R rabbit monoclonal antibody supports investigations in immunology, inflammation, and oncology, areas where high-quality reagents are in constant demand. Our team can assist with sourcing rare or custom targets, ensuring seamless access to the materials you need from concept to clinic.