Anti-IL22 Rabbit Polyclonal Antibody for WB, ELISA - Q9JJY9

Core Product Specifications and Parameters

Biological Background: IL22 Function and Localization

• IL22, also known as IL-10-related T-cell-derived-inducible factor (IL-TIF) or IL-22a, is a ~20 kDa secreted glycoprotein of the IL-10 cytokine family, characterized by a three-dimensional structure stabilized by disulfide bonds.
• Plays a critical role in modulating tissue responses during inflammation, contributing to host defense and tissue pathology.
  Related references: PMID:33852830, PMID:35525330
• Essential for the regeneration of epithelial cells to maintain barrier function after injury and for preventing further tissue damage.
  Related references: PMID:33912578
• Signals via the IL22RA1/IL10RB receptor complex to trigger JAK1/TYK2/STAT3 cascades and downstream ERK1/2 and PI3K/AKT pathways, driving cell survival and proliferation. Promotes phosphorylation of GSK3B at Ser-9 and CTTN, facilitating epithelial cell spreading.
  Related references: PMID:24742671
• Uniquely among cytokines, IL-22 has no direct effect on immune cells but acts exclusively on non-immune epithelial and stromal cells to maintain tissue homeostasis.

Experimental Guidance and Technical Tips

• Immunogen design: The immunogen corresponds to amino acids 34-179 of mouse IL22 (NP_058667.1), representing the mature form of the protein after cleavage of the signal peptide. This design targets the biologically active secreted cytokine, suitable for detecting endogenous IL22 in relevant biological samples.
• Western blot tips: For Western blot, consider using lysates from activated T cells or stimulated epithelial cells (e.g., mouse intestinal organoids or keratinocytes), as IL22 expression is typically induced under inflammatory conditions. The predicted molecular weight is ~20 kDa, but glycosylation may result in slightly higher bands. Validate antibody specificity with positive and negative controls.
• ELISA tips: For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent. Pairing with a monoclonal antibody may enhance specificity and sensitivity. Ensure proper blocking and dilution optimization for the intended sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, providing high-quality antibodies like this anti-IL22 rabbit polyclonal antibody to support research and diagnostic development in cytokine biology and epithelial barrier function. Our comprehensive portfolio includes validated antibody pairs, optimized polyclonal antibodies, and rare-target raw-material sourcing to accelerate your assay development from concept to clinic.