Anti-IL22 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9GZX6

Core Product Specifications and Parameters

Biological Background: IL22 Function and Localization

• IL22 is a cytokine that modulates tissue responses during inflammation and is essential for epithelial cell regeneration and barrier maintenance after injury. Unlike most cytokines, it has no direct effect on immune cells. Related references: PMID:17204547
• Signals through a heterodimeric receptor composed of IL22RA1 (specific, on non-immune cells) and IL10RB (shared subunit). Related references: PMID:10875937 PMID:18599299
• Receptor ligation activates JAK1 and TYK2 kinases, leading to STAT3, ERK1/2, and PI3K/AKT pathway activation, promoting cell survival and proliferation. Related references: PMID:25793261 PMID:31311100
• Promotes phosphorylation of GSK3B at Ser-9 and CTTN (cortactin), and promotes epithelial cell spreading (By similarity).
• The protein is secreted and has a molecular weight of approximately 20 kDa (20011 Da). Keywords: Cytokine, Disulfide bond, Glycoprotein, Secreted.
• Also known as Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor (IL-TIF), TIFa, IL-21, IL-22, ILTIF, IL-D110, zcyto18, TIFIL-23.

Experimental Guidance and Technical Tips

• The immunogen spans the mature IL22 sequence (aa 34-179), which may enable detection of both full-length and cleaved forms in Western blot.
• For Western blot, the predicted band is around 20 kDa; consider using a reducing SDS-PAGE to resolve the monomeric form.
• For IF/ICC, optimize fixation and permeabilization protocols as IL22 is a secreted cytokine; validate with appropriate controls.
• When using ELISA, validate the antibody pair with the recombinant immunogen or native protein in relevant sample matrices.
• Cross-reactivity with mouse and rat IL22 suggests sequence homology in the immunogen region; however, validate antibody performance in each species' system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. For IL22 research, we offer validated antibody pairs, optimized monoclonal antibodies like this anti-IL22 clone, and bulk ancillary reagents to support immunoassay development. Our sourcing capabilities cover rare targets and customized production, ensuring you can move from concept to clinic with confidence.