Anti-IL21 Rabbit Monoclonal Antibody for WB and ELISA - Q9HBE4

Core Product Specifications and Parameters

Biological Background: IL21 Function and Localization

• Interleukin-21 (IL21), also referred to as Za11, is a cytokine encoded by the IL21 gene and belongs to the gamma-chain receptor cytokine family.
• IL21 exerts immunoregulatory activity by promoting the transition between innate and adaptive immunity and inducing the production of IgG1 and IgG3 in B-cells (By similarity).
• Together with IL6, IL21 controls the early generation of T follicular helper (Tfh) cells and is essential for effective antibody responses to acute viral infections (By similarity).
• In synergy with IL15, IL21 may enhance the proliferation and maturation of natural killer (NK) cells, and when combined with IL15 and IL18, it stimulates interferon gamma production in T-cells and NK cells. Related references: PMID:11081504, PMID:15178704
• During T-cell mediated immune responses, IL21 may inhibit dendritic cell (DC) activation and maturation (By similarity).
• IL21 is a secreted glycoprotein containing a disulfide bond, and alternative splicing generates additional isoforms; it is predominantly expressed in activated CD4-positive T-cells, not in CD8-positive T-cells, B-cells, or monocytes.
• The mature protein has a predicted molecular weight of approximately 18.6 kDa (UniProt) and commonly migrates at 17–18 kDa in SDS-PAGE, consistent with the observed band sizes.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 25-162 of mature human IL21, enabling recognition of the secreted cytokine; verify reactivity in samples derived from activated CD4+ T-cells or other expression systems.
• For Western blotting, a band at 17–18 kDa is expected; consider using low-molecular-weight markers and optimizing transfer conditions. Due to potential glycosylation, slight mass shifts may occur.
• In ELISA applications, this monoclonal antibody may serve as a capture or detection reagent; however, pairing compatibility should be validated with the intended sandwich format.
• Since IL21 can be expressed at low levels, concentrate cell supernatants or enrich specific cell populations to improve detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for diagnostic and research applications. For projects targeting IL21 or other cytokines, we provide tailored support to rapidly connect you with qualified raw materials from early development through commercialization. Our streamlined supply network assists in reducing lead times and securing rare-target reagents, empowering your immunology and diagnostic programs.