Anti-IL2 Rabbit Monoclonal Antibody for WB, ELISA - P04351

Core Product Specifications and Parameters

Biological Background: IL2 Function and Localization

• IL2, also known as T-cell growth factor (TCGF), is a cytokine essential for T-cell proliferation and immune response regulation. Related references: PMID:14614860, PMID:9814585

• It binds to either high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122, IL2RG/CD132) or low-affinity dimeric IL-2R, initiating JAK/STAT, PI3K, and MAPK signaling pathways. Related references: PMID:14614860, PMID:27018889

• Functions as a T-cell growth factor, enhances NK-cell cytolytic activity, and promotes B-cell proliferation and immunoglobulin production. Related references: PMID:14614860

• Plays a critical role in the maintenance of immune tolerance by controlling regulatory T-cell survival and proliferation, and participates in effector T-cell differentiation (Th1, Th2, Th17) and memory CD8+ T-cell homeostasis. Related references: PMID:14614860, PMID:9814585

• Subcellular localization: Secreted. Tissue specificity: Produced by activated T-cells, NK cells, and dendritic cells, but not macrophages upon bacterial stimulation.

• Keywords indicate it is a glycoprotein with disulfide bonds, part of adaptive immunity, and acts as a growth factor.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 21–169 of mouse IL2. This region excludes the signal peptide and covers the mature cytokine. Consider this when selecting positive control samples or recombinant standards.
• For WB, a band near 19 kDa is expected under reducing conditions. Ensure sample preparation includes sufficient reducing agent and boiling, as IL2 contains disulfide bonds.
• ELISA application may require optimization of coating conditions and detection antibody pairing. Validate in the relevant mouse immune cell or serum sample system.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supports diagnostic developers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for immune response research. For IL2-targeted assay development, CamelBio offers comprehensive sourcing solutions, including rare-target raw materials, to streamline your project from concept to clinic.