Anti-IL1β Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P10749

Core Product Specifications and Parameters

Biological Background: IL1β Function and Localization

• Interleukin-1 beta (IL1β) is a potent pro-inflammatory cytokine belonging to the IL-1 family. It is produced as an inactive precursor (pro-IL1β) and cleaved to its mature active form by caspase-1.
• IL1β mediates pleiotropic effects including induction of prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
• It promotes Th17 differentiation of T-cells and synergizes with IL12 to induce IFNG synthesis from T-helper 1 (Th1) cells.
• IL1β plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
• Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released through the gasdermin-D (GSDMD) pore.
• Subcellular localization includes cytoplasm, cytosol, lysosome, and secreted extracellularly via exosomes.
• Expressed in activated macrophages at the protein level.
• Key functional tags: cytokine, inflammatory response, mitogen, pyrogen, and secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 118-269 of mouse IL1β, which covers the mature secreted form. This design is expected to recognize the active cytokine in various species.
• For Western blot (WB), consider using reducing conditions and appropriate positive controls such as LPS-stimulated macrophage lysates; the predicted molecular weight is approximately 31 kDa, but post-translational modifications may affect migration.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization may be necessary. Validate in tissue samples known to express IL1β.
• ELISA applications may require pairing with a detection antibody and optimization of capture/detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials, including validated monoclonal antibodies like this anti-IL1β clone. For projects targeting inflammation, immunology, or infectious disease, we can support your workflow with optimized antibody pairs, bulk ancillary reagents, and rare target sourcing—helping you accelerate from concept to clinic efficiently.