Anti-IL1β Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P10749

Core Product Specifications and Parameters

Biological Background: IL1β Function and Localization

• Interleukin-1 beta (IL1β) is a potent pro-inflammatory cytokine belonging to the interleukin-1 family, encoded by the Il1b gene in mouse (Gene ID: 16176, UniProt P10749). The protein is synthesized as a 31 kDa precursor that is cleaved to generate a mature secreted form.
• IL1β was initially identified as the major endogenous pyrogen, capable of inducing fever. It stimulates prostaglandin synthesis, promotes neutrophil influx and activation, and regulates both T-cell and B-cell functions, driving adaptive immune responses.
• IL1β plays a central role in the inflammatory cascade: it synergizes with IL12 to induce interferon-gamma (IFNG) production from Th1 cells, and promotes Th17 differentiation. It also facilitates angiogenesis by inducing VEGF production in combination with TNF and IL6.
• The mature form of IL1β is released extracellularly via a non-classical secretion pathway. Upon inflammasome activation and pyroptosis, mature IL1β passively exits through gasdermin-D (GSDMD) pores in the plasma membrane, linking innate immune sensing to inflammation.
• Subcellularly, IL1β is found in the cytosol, lysosomes, and is actively secreted. It is also associated with extracellular exosomes, indicating multiple secretion routes (UniProt: SUBCELLULAR LOCATION).
• Expression of IL1β is tightly regulated and predominantly observed in activated macrophages at the protein level, consistent with its role in innate immunity (UniProt: TISSUE SPECIFICITY).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 118-269 of mouse IL1β precursor (NP_032387.1), covering the mature IL1β region. The antibody may recognize both the ~31 kDa precursor and the ~17 kDa mature form depending on the sample and activation state. It is advisable to run appropriate molecular weight markers and positive controls (e.g., LPS-stimulated macrophage lysates) to identify the specific bands.
• For Western blot (WB), use a reducing SDS-PAGE and standard transfer protocols. A band around 31 kDa is expected for the full-length precursor, while a band at ~17 kDa may appear in secreted or cleaved samples.
• In immunofluorescence/ICC (IF/ICC), include stimulation controls (e.g., LPS ± ATP) to induce IL1β expression and secretion, as basal levels may be low. Co-staining with organelle markers may help visualize its cytosolic or secreted localization.
• For ELISA, consider pairing this antibody with a suitable detection antibody recognizing a distinct epitope if used as a capture reagent. Validate the assay with recombinant mouse IL1β standards and biological samples.
• Cross-reactivity is confirmed for mouse; perform a species-specific validation if used on other species. Endogenous mouse IgG may produce background in mouse tissues; using anti-rabbit secondary antibodies and appropriate blocking can mitigate this.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline the procurement of high-quality IVD raw materials. This IL1β rabbit monoclonal antibody is one example of our comprehensive portfolio covering key immunological targets, and we support projects from concept to clinic. In addition to validated antibody clones, we provide one-stop access to customized monoclonal and polyclonal antibody development, bulk ancillary reagents, and rare-target sourcing—helping you accelerate your inflammation and cytokine research workflows with reliable supply solutions.