Anti-IL1β Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P01584

Core Product Specifications and Parameters

Biological Background: IL-1 beta Function and Localization

• Interleukin-1 beta (IL-1β), also known as catabolin, is a pro-inflammatory cytokine encoded by the IL1B gene and belongs to the IL-1 family.
• Functions as a major endogenous pyrogen, inducing prostaglandin synthesis, fever, and systemic inflammatory responses (PubMed:3920526).
  Related references: PMID:3920526
• Promotes Th17 differentiation of T-cells and synergizes with IL-12 to stimulate IFN-γ production from Th1 cells, linking innate and adaptive immunity (PubMed:10653850).
  Related references: PMID:10653850
• Participates in angiogenesis by inducing VEGF expression in collaboration with TNF and IL-6, contributing to tissue remodeling and tumor progression (PubMed:12794819).
  Related references: PMID:12794819
• Plays a critical role in pyroptosis-mediated inflammation: the mature form is released extracellularly through gasdermin-D pores upon inflammasome activation (PubMed:33377178, PubMed:33883744).
  Related references: PMID:33377178 PMID:33883744
• Serves as an innate immune sensor for Streptococcus pyogenes skin infection; bacterial SpeB protease cleaves pro-IL-1β, activating a protective inflammatory response (PubMed:28331908).
  Related references: PMID:28331908
• Localizes to the cytoplasm, cytosol, and is actively secreted; it is also found in lysosomes and extracellular exosomes, consistent with its regulated release.
• Expressed predominantly in activated monocytes and macrophages, with tight regulation to prevent systemic toxicity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 170-269 of human IL-1β (NP_000567.1), covering the C-terminal portion of the pro-form. This region is retained in the mature cytokine; the antibody may recognize both the 31 kDa precursor and the 17 kDa mature form in denaturing Western blots, depending on sample preparation.
• For Western blotting, consider using reducing conditions and a broad molecular weight range to visualize potential precursor and processed forms. Validate detection in relevant cell lysates or stimulated monocyte/macrophage samples.
• Immunofluorescence (IF-P) may require optimized fixation and permeabilization to detect cytosolic IL-1β. Include appropriate controls to distinguish secreted versus intracellular pools.
• In ELISA applications, this monoclonal antibody may serve as a capture or detection reagent. Pairing with a second antibody targeting a distinct epitope is recommended for sandwich immunoassays.
• Given the potent pro-inflammatory nature of IL-1β, experimental systems should include stimulation controls (e.g., LPS with or without inflammasome activators) to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies diagnostic development by offering a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this IL-1β clone for inflammation and infectious disease research. Our technical and supply teams support customers with antibody pairs, ancillary reagents, and custom sourcing for rare targets—helping to streamline your transition from concept to clinical application.