Anti-IL1RA Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P18510

Core Product Specifications and Parameters

Biological Background: IL1RA Function and Localization

• IL1RA (Interleukin-1 receptor antagonist, also known as IL1RN, IL1F3, IRAP) is a member of the interleukin-1 cytokine family that functions as a competitive inhibitor of IL1A and IL1B signaling.
• It binds to the IL1 receptor without inducing a cellular response, thereby blocking the proinflammatory actions of IL1 and protecting against immune dysregulation and systemic inflammation triggered by pathogens and other innate stimulatory agents.
• The IL1RN gene undergoes alternative splicing, producing secreted and intracellular isoforms; the intracellular form (icIL-1RA) is predominantly expressed in epithelial cells.
• Subcellular localization includes secretion and cytoplasmic distribution, consistent with its role as an extracellular cytokine antagonist and potentially intracellular regulatory functions.
• Post-translational modifications include glycosylation and the presence of disulfide bonds, which may influence protein stability and activity.
• The mature protein has a molecular weight of approximately 20 kDa (20,055 Da as per UniProt), which aligns with the 20kDa band detected in applications.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 24–177 of human IL1RA, covering the mature secreted form; it is expected to recognize both secreted and intracellular isoforms.
• For Western blot (WB), a band at approximately 20 kDa is anticipated; however, glycosylation may cause a slight shift. Consider deglycosylation experiments to confirm specificity.
• If using for immunofluorescence (IF/ICC or IF-P), verify fixation and permeabilization conditions, as IL1RA can be secreted or intracellular.
• The antibody cross-reacts with mouse and rat, but validation in these species is recommended by testing relevant tissue or cell lysates alongside appropriate controls.
• For ELISA, assess pairing with a detection antibody and optimize coating conditions to achieve desired sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials and technical services from concept to clinic. This IL1RA antibody is part of our inflammation and cytokine portfolio, and we can provide validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Contact us to discuss how we can streamline your IVD development with reliable raw-material supply.