Anti-IL18BP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95998

Core Product Specifications and Parameters

Biological Background: IL18BP Function and Localization

• Interleukin-18-binding protein (IL18BP) is a secreted protein that acts as a high-affinity inhibitor of the proinflammatory cytokine IL-18, thereby suppressing early TH1 cytokine responses.
• The protein exists as multiple isoforms due to alternative splicing; isoform A (the canonical sequence) binds IL-18 and blocks its interaction with the IL-18 receptor.
• IL18BP contains an immunoglobulin-like domain and is a glycoprotein with conserved disulfide bonds, contributing to its structural stability and binding function.
• It is strongly expressed in heart, lung, placenta, and spleen, reflecting its role in modulating inflammatory responses in diverse tissues.
• As a secreted protein, IL18BP is released into the extracellular space where it neutralizes circulating IL-18.
• The recombinant form of IL18BP, known as tadekinig-alfa, has been investigated for therapeutic modulation of IL-18-mediated diseases.

Experimental Guidance and Technical Tips

• For Western blot, a band around 21 kDa may be observed under reducing conditions; however, glycosylation can increase the apparent molecular weight, so validate with a positive control lysate from tissues such as placenta or spleen.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider using cells or tissues with known high IL18BP expression (e.g., placental or lung-derived lines) and optimize permeabilization protocols to detect the secreted protein.
• For ELISA applications, this polyclonal antibody can be used as a detection reagent; ensure appropriate coating and blocking conditions, and pair with a suitable capture antibody if developing a sandwich assay.
• The antibody is raised against amino acids 31-194 of human IL18BP and has been shown to cross-react with mouse; when working with mouse samples, verify the expected reactivity in your specific experimental system.
• As a polyclonal antibody, it may recognize multiple isoforms of IL18BP generated by alternative splicing; consider this when interpreting results in tissues with differential isoform expression.

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CamelBio is your comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents like this anti-IL18BP polyclonal antibody for inflammation and cytokine studies. We offer customized supply solutions including validated antibody pairs, bulk ancillary reagents, and rare target raw materials to streamline your assay development from concept to clinic.