Anti-IL18 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q14116

Core Product Specifications and Parameters

Biological Background: IL-18 Function and Localization

• Interleukin-18 (IL-18), also known as interferon-gamma-inducing factor (IGIF), Iboctadekin, or IL-1 gamma, is encoded by the IL18 gene (aliases: IGIF, IL1F4). It belongs to the IL-1 cytokine family and exists in multiple isoforms due to alternative splicing.
• IL-18 is a pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses, and synergizes with IL-12 to induce interferon-gamma (IFN-gamma) synthesis (PubMed:10653850).
  Related references: PMID:10653850
• Upon binding to its receptor complex IL18R1 and IL18RAP, IL-18 activates NF-kappa-B signaling, triggering the synthesis of various inflammatory mediators (PubMed:14528293, PubMed:25500532, PubMed:37993714).
  Related references: PMID:14528293 PMID:25500532 PMID:37993714
• The mature, biologically active form of IL-18 is released into the extracellular space through gasdermin-D (GSDMD) pores during pyroptosis, linking inflammation to programmed cell death (PubMed:33883744).
  Related references: PMID:33883744
• Subcellularly, IL-18 is located in the cytoplasm and cytosol, and is ultimately secreted.
• Tissue expression: IL-18 is expressed in ovarian carcinoma but is undetectable in normal ovarian epithelial cells. It is resistant to proteolytic activation by caspase-1 and -4.
• Additional keywords from UniProt: 3D-structure, alternative splicing, direct protein sequencing, and proteomics identification confirm its status as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen (aa 37-193) corresponds to the mature IL-18 protein, targeting the active cytokine region, which should provide specificity for the biologically relevant form.
• This polyclonal antibody has been applied in WB, IP, and ELISA. For western blot, consider reducing SDS-PAGE conditions due to the predicted molecular weight of ~22 kDa.
• Given the restricted expression in ovarian carcinoma, validate the antibody in relevant human cancer cell lines or tissue samples where IL-18 is known to be upregulated.
• For ELISA and IP, optimize antibody concentrations based on your specific assay systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for one-stop IVD raw-material sourcing, technical services, and consulting, supporting every stage from concept to clinic. This anti-IL18 polyclonal antibody is ideal for applications in inflammation, oncology, and pyroptosis research, and we can supply it along with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. Let CamelBio bridge your procurement needs and accelerate your diagnostic development.