Anti-IL18 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P70380

Core Product Specifications and Parameters

Biological Background: IL18 Function and Localization

• Interleukin-18 (IL18), also known as interferon gamma-inducing factor (IGIF) and interleukin-1 gamma (IL-1 gamma), is a pro-inflammatory cytokine encoded by the Il18 gene in mice.
• It plays a key role in epithelial barrier repair and orchestrates Th1 and NK cell immune responses. Related references: PMID:26638072 PMID:26638073
• IL18 binding to IL18R1 and IL18RAP forms a signaling complex that activates NF-kappa-B, promoting the synthesis of inflammatory mediators.
• It synergizes with IL12 to stimulate IFNG production from Th1 and NK cells.
• During pyroptosis, mature IL18 is released through gasdermin-D (GSDMD) pores into the extracellular milieu. Related references: PMID:30392956
• Subcellularly, IL18 is found in the cytoplasm and is secreted upon activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature form of mouse IL18 (amino acids 36–192), suggesting the antibody detects the cleaved, active cytokine; validate in the relevant sample system.
• For Western blot, consider using reducing conditions and check for bands around 22 kDa; be aware that IL18 may exist as a precursor and mature form.
• For IHC-P, antigen retrieval protocols may need optimization; consider testing on positive control tissues with known IL18 expression.
• This antibody cross-reacts with human and rat IL18; for multispecies studies, verify specificity in each species’ sample.
• Pair with appropriate secondary antibodies and detection systems for ELISA; validate with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal antibodies like this anti-IL18 pAb, and bulk ancillary reagents to support diagnostic development. Our platform streamlines procurement from concept to clinic, including rare target raw-material sourcing for immunology and inflammation research.