Anti-IL17RE Rabbit Polyclonal Antibody for WB, ELISA - Q8NFR9

Core Product Specifications and Parameters

Biological Background: IL17RE Function and Localization

• IL17RE (Interleukin-17 receptor E) is a specific functional receptor for the cytokine IL17C, mediating signaling through NF-kappa-B and MAPK pathways and requiring the adaptor TRAF3IP2/ACT1 for downstream signaling.
• It plays a crucial role in innate immunity to bacterial pathogens, particularly in mucosal tissues.
• Alternatively spliced isoforms exist: isoforms 2 and 4 may be cytoplasmic inactive or dominant active forms, while isoforms 3 and 5 may act as soluble decoy receptors.
• Subcellular localization includes the cell membrane, cytoplasm, and secretion; the protein possesses a signal peptide and a transmembrane domain.
• Predominantly expressed in mucosal tissues, with high levels in keratinocytes and colon epithelial cells; very low expression in dermal fibroblasts, and also found in various tumor cell lines.
• The protein is a glycoprotein, and its UniProt-calculated molecular weight is approximately 74.8 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the predicted extracellular domain (amino acids 24–260); the antibody may recognize both full-length membrane-bound and secreted isoforms; confirm detection specificity in your sample system.
• For Western blot, anticipate a major band near 75 kDa, though glycosylation may cause migration shifts; use appropriate molecular weight markers and consider deglycosylation controls.
• Cross-reactivity is reported for human and mouse; validate in mouse tissue lysates known to express IL17RE, such as keratinocytes or colon epithelial cells.
• For ELISA, pre-test with recombinant protein standards and consider blocking conditions to minimize background, especially when analyzing samples with potential soluble decoy isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to high-quality IVD raw materials. For IL17RE and related innate immunity targets, we supply validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents—supporting assay development from early-phase research through clinical application. Our one-stop model simplifies procurement, helping you accelerate project timelines.