Anti-IL17RA Rabbit Monoclonal Antibody for WB, ELISA - Q96F46

Core Product Specifications and Parameters

Biological Background: Interleukin-17 receptor A Function and Localization

• IL17RA (Interleukin-17 receptor A) is a glycosylated type I transmembrane protein and member of the IL-17 receptor family. Alternative splicing generates a secreted isoform.
• As a receptor for the pro-inflammatory cytokines IL17A and IL17F, IL17RA forms a heterodimer with IL17RC to initiate signaling. Ligand binding recruits the adaptor TRAF3IP2 (ACT1), leading to TRAF6-mediated activation of NF-κB and MAPK cascades, which drive expression of cytokines, chemokines, and antimicrobial peptides. Related references: PMID:17911633, PMID:9367539, PMID:19838198, PMID:16785495, PMID:18684971
• In antimicrobial host defense, IL17RA signaling is critical for neutrophil recruitment and activation at infection sites. It also stimulates mucosal epithelial cells to produce β-defensins (DEFB1, DEFB103A, DEFB104A), limiting microbial entry.
• Within secondary lymphoid organs, IL17RA promotes germinal center formation by regulating B cell chemotaxis to CXCL12 and CXCL13, enhancing B cell retention, somatic hypermutation, and plasma cell differentiation.
• IL17RA contributes to antiviral immunity: it enhances T cell cytotoxicity against West Nile virus and drives B-1a B cell differentiation for influenza virus clearance, providing an early IgM antibody response.
• Beyond the classic IL17A/F ligands, IL17RA partners with IL17RE to mediate IL17C signaling, broadening its cytokine repertoire. Related references: PMID:21993848
• Additionally, IL17RA acts as a receptor for the SARS-CoV-2 ORF8 protein, which hijacks the IL17 pathway to amplify pro-inflammatory signaling.
• IL17RA is widely expressed and localizes primarily to the cell membrane, with a soluble secreted form detectable.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the intracellular C‑terminal region (aa 767–866) of human IL17RA, a segment that is partially conserved in mouse and rat, supporting the observed cross‑reactivity.
• For western blotting, expect a band near the theoretical weight of 96 kDa; post‑translational modifications such as N‑linked glycosylation may cause a slight upward shift. Always validate specificity using IL17RA knockout or knockdown cell lysates.
• In ELISA applications, this rabbit monoclonal antibody can serve as a detection reagent when paired with a capture antibody that recognizes a spatially distinct epitope; verify compatibility with your specific sample matrix and assay format.
• The monoclonal nature of this antibody ensures lot‑to‑lot consistency, making it a reliable choice for quantitative immunoassays and long‑term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research labs with integrated access to IVD raw materials, including validated monoclonal antibodies like this anti‑IL17RA clone, meeting the demands of inflammatory and infectious disease assay development. Through our global sourcing network, we can also supply optimized antibody pairs, bulk ancillary reagents, and custom procurement of rare‑target raw materials, helping you streamline the transition from research to commercial immunoassay production.