Anti-IL17A Rabbit Monoclonal Antibody for WB, ELISA - Q62386

Core Product Specifications and Parameters

Biological Background: IL-17A Function and Localization

• IL-17A is a secreted effector cytokine of innate and adaptive immunity, crucial for antimicrobial host defense and tissue integrity maintenance. Related references: PMID:18025225 PMID:19144317 PMID:26431948
• It signals through the IL17RA-IL17RC receptor complex, activating NF-kappa-B and MAPK pathways to induce pro-inflammatory cytokines, chemokines, and antimicrobial peptides. Related references: PMID:16200068 PMID:17911633 PMID:19144317
• As a signature cytokine of Th17 cells, IL-17A drives neutrophil activation and recruitment to infection sites, partly via CXCL1 and CXCL5 induction in airway epithelium. Related references: PMID:18025225 PMID:27923703
• It contributes to germinal center formation by regulating B cell chemotaxis to CXCL12 and CXCL13, influencing antibody responses. Related references: PMID:18157131
• IL-17A is also produced by gamma-delta T cells, iNKT cells, and innate lymphoid cells, mediating early neutrophilic inflammation and bacterial clearance. Related references: PMID:17372004 PMID:20364087 PMID:28709803 PMID:17470641 PMID:23255360
• In antiviral defense, IL-17A enhances T cell cytotoxicity, promotes B cell recruitment and IgM production, aiding clearance of viruses like influenza and West Nile virus. Related references: PMID:21946434 PMID:26735852 PMID:27795421
• IL-17A is secreted and exhibits tissue-specific expression in Th17 cells, innate lymphoid cells, gamma-delta T cells, and iNKT cells.

Experimental Guidance and Technical Tips

• For Western blot, start with a dilution of 1:500–1:2000 and validate in mouse tissue lysates from Th17-polarized cells or inflammatory models (e.g., lung, intestine).
• For ELISA, test as a coating antibody (e.g., 1–10 µg/mL) paired with a suitable detection antibody against mouse IL-17A, using recombinant IL-17A as standard.
• The immunogen covers the mature peptide (aa 26–158); the antibody likely recognizes both monomeric and dimeric forms. Consider reducing and non-reducing SDS-PAGE to assess.
• Cross-reactivity is limited to mouse; not recommended for human or rat samples unless experimentally validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for immunoassay development. This anti-IL-17A antibody supports research and diagnostic projects in inflammatory and autoimmune diseases. Contact us for rare target raw-material sourcing and custom development support.