Anti-IL13RA2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14627

Core Product Specifications and Parameters

Biological Background: IL13RA2 Function and Localization

• IL13RA2 (interleukin-13 receptor subunit alpha-2), also designated CD213A2 or IL-13-binding protein (IL-13BP), is a high-affinity receptor for interleukin-13 (IL-13).
• It functions primarily as a decoy receptor, inhibiting IL-13- and IL-4-mediated signal transduction through the JAK-STAT pathway, thereby modulating immune and inflammatory responses. Related references: PMID:11861389 PMID:17030238
• In addition to its decoy role, IL13RA2 can act as a signaling receptor in an alternative pathway involving AP-1, ultimately leading to the production of transforming growth factor beta-1 (TGFB1). Related references: PMID:16327802
• Unlike the related IL13RA1 chain, IL13RA2 lacks the cytoplasmic motifs necessary for JAK-STAT signaling, underpinning its decoy mechanism.
• The protein is localized predominantly at the cell membrane and contains a signal peptide, three extracellular fibronectin type-III repeats, a single transmembrane helix, and a short cytoplasmic tail.
• Structurally, IL13RA2 is a glycoprotein with conserved disulfide bonds, features characteristic of the type I cytokine receptor family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 27-343), suggesting the antibody may recognize the native receptor on live cells in addition to denatured forms.
• For WB, a band at approximately 44 kDa is expected; however, glycosylation may result in a higher apparent molecular weight. Validate in the relevant sample system.
• Consider optimizing antigen retrieval and fixation conditions for IHC-P, as membrane proteins can be sensitive to processing.
• Verify cross-reactivity in mouse and rat tissues, and note that IL13RA2 expression may be upregulated under certain pathological conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials and technical support, covering every stage from concept to clinic. This anti-IL13RA2 polyclonal antibody is suitable for investigations into IL-13 signaling, immune regulation, and cancer biology. We also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare-target raw materials to accelerate your assay development.