Anti-IL13RA1 Monoclonal Antibody for WB, IHC-P, ELISA - P78552

Core Product Specifications and Parameters

Biological Background: IL13RA1 Function and Localization

• Interleukin-13 receptor subunit alpha-1 (IL13RA1) is a low-affinity receptor for interleukin-13 (IL-13).
• Together with interleukin-4 receptor alpha (IL4RA), it forms a functional receptor complex for IL-13, initiating downstream signaling.
• IL13RA1 also acts as an accessory protein for interleukin-4 (IL-4) signaling, but cannot substitute for IL2RG in enhancing IL-2 binding.
• The protein is a single-pass type I membrane receptor with an extracellular domain containing multiple repeats, a transmembrane helix, and a short cytoplasmic tail.
• IL13RA1 is ubiquitously expressed, with highest mRNA levels in heart, liver, skeletal muscle, and ovary, and lower levels in brain, lung, and kidney; it is also detected in B-cells, T-cells, and endothelial cells.
• Alternative splicing generates multiple transcript variants encoding different isoforms.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation.
• The protein is recognized as cancer/testis antigen 19 (CT19), indicating potential expression in some tumors.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1–100) of human IL13RA1; consider that the epitope lies within the extracellular domain when designing assays.
• For Western blotting, the predicted molecular weight is 49 kDa; however, glycosylation may lead to a higher apparent molecular weight, so validate band size in your cell or tissue lysates.
• In immunohistochemistry (paraffin-embedded tissues), antigen retrieval steps and antibody dilution may require optimization due to the membrane localization of the target.
• Given the ubiquitous expression of IL13RA1, include appropriate positive and negative controls tailored to your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality antibodies and reagents. This anti-IL13RA1 rabbit monoclonal antibody supports investigations into cytokine signaling and cancer biomarker research. Beyond this product, CamelBio provides validated antibody pairs, bulk ancillary reagents, and custom procurement for rare targets to accelerate your assay development process.