Anti-IL12RB2 Polyclonal Antibody for WB, ELISA - Q99665

Core Product Specifications and Parameters

Biological Background: IL12RB2 Function and Localization

• IL12RB2 encodes the interleukin-12 receptor subunit beta-2, a single-pass type I transmembrane protein that serves as the signaling component of the IL-12 receptor complex.
• Upon IL-12 binding, IL12RB2 couples to the JAK2/STAT4 signaling pathway, promoting the proliferation of T-cells and NK cells and strongly enhancing IFN-gamma production.
• This signaling drives the differentiation of T-cells toward the Th1 phenotype, playing a critical role in cell-mediated immunity.
• Alternative splicing produces multiple isoforms; isoform 2 is expressed at similar levels in both naive and activated T-cells.
• The protein localizes to the cell membrane and features domains including a signal peptide, extracellular ligand-binding repeats, a transmembrane helix, and an intracellular tail.
• Post-translational modifications include glycosylation and phosphorylation, consistent with its role in signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 420–620 of human IL12RB2 (NP_001550.1), located in the extracellular domain. This region is suitable for targeting the receptor in native and denatured conditions.
• For Western blotting, a band at approximately 97 kDa is expected. Validate specificity using positive controls such as IL-12-stimulated T-cells or NK cells.
• For ELISA, consider using recombinant human IL12RB2 protein as the coating antigen, and confirm detection in relevant sample matrices.
• As cross-reactivity with mouse and rat is indicated, species-specific protocols may require optimization; validate in the respective tissue or cell lysates.
• To maintain antibody stability, avoid repeated freeze-thaw cycles and store at recommended conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering diagnostic manufacturers and research institutions streamlined access to validated antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This anti-IL12RB2 polyclonal antibody supports research in immunology and cell signaling, and our team can assist with complementary reagent procurement—from optimized antibody pairs to assay components—to accelerate your project from concept to clinic.