Anti-IL12RB1 Rabbit Polyclonal Antibody for WB, ELISA - P42701

Core Product Specifications and Parameters

Biological Background: IL12RB1 Function and Localization

• IL12RB1 encodes the interleukin-12 receptor subunit beta-1, a type I transmembrane protein belonging to the cytokine receptor family.
• It binds interleukin-12 (IL-12) with low affinity and associates with IL12RB2 to form a high-affinity functional receptor for IL-12.
• IL12RB1 also pairs with IL23R to constitute the interleukin-23 receptor, which mediates IL-23 signal transduction.
• Ligand engagement triggers the Jak-Stat signaling cascade, linking extracellular cytokine signals to intracellular responses.
• The receptor localizes primarily to the cell membrane and features extracellular fibronectin type III-like domains, disulfide bonds, and N-glycosylation.
• Alternative splicing produces multiple isoforms, and missense variants are linked to primary immunodeficiency diseases (IMD30).
• The canonical protein has a calculated molecular weight of approximately 73 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 25–305) of human IL12RB1, making the antibody suitable for detecting the receptor’s ectodomain.
• For WB, consider using lysates from IL-12/IL-23-responsive cells (e.g., activated T cells, NK cells) of human, mouse, or rat origin, and optimize loading amounts and blocking conditions.
• In ELISA, ensure the coating antigen or capture format includes the immunogen region; pre-adsorption controls may help confirm specificity.
• Validate cross-reactivity in your specific model system and include appropriate positive and negative controls to interpret species-dependent signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized IVD raw-material partner, offering diagnostic manufacturers and research teams reliable access to antibodies, validated pairs, and bulk ancillary reagents. This anti-IL12RB1 polyclonal antibody supports development of immunoassays targeting the IL-12/IL-23 signaling axis in immune function and disease. For scaled production or rare target sourcing needs, CamelBio provides tailored supply solutions to bridge your concept-to-clinic workflow.