Anti-IL12B Rabbit Polyclonal Antibody for WB, ELISA - P43432

Core Product Specifications and Parameters

Biological Background: IL12B Function and Localization

• IL12B encodes the p40 subunit shared by the heterodimeric cytokines interleukin-12 (IL-12) and interleukin-23 (IL-23).
• In IL-12, p40 pairs with p35 (IL12A) to form a cytokine that acts as a growth factor for activated T and NK cells, enhances their lytic activity, and stimulates IFN-gamma production by resting PBMC.
• In IL-23, p40 associates with IL23A p19; the resulting cytokine binds a receptor complex of IL12RB1 and IL23R, activating the Jak-Stat signaling cascade to preferentially stimulate memory T-cells and promote pro-inflammatory cytokine production.
• IL-23 participates in acute responses to infection in peripheral tissues, often acting in concert with IL-17, and has been implicated in driving autoimmune inflammation and tumorigenesis.
• The protein is secreted and contains an immunoglobulin-like domain, multiple disulfide bonds, and N-linked glycosylation sites.
• A signal peptide directs its secretion, and it belongs to the type I cytokine receptor family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 23–335 of mouse IL12B, spanning the mature p40 subunit without the signal peptide; this design targets the conserved functional domain and supports cross-reactivity with human IL12B.
• For WB, a band near 38 kDa is expected; consider using a recombinant IL12B protein as positive control and validate detection in relevant mouse and human tissue or cell lysates.
• In ELISA, pair this polyclonal antibody with a well-matched capture/detection partner after confirming performance in your specific sample matrix and standard curve setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with end-to-end access to IVD raw materials, from concept to clinic. This IL12B rabbit polyclonal antibody is part of our cytokine and immuno-oncology portfolio, supported by validated antibody pairs, optimized monoclonal/polyclonal options, bulk ancillary reagents, and custom sourcing for rare or challenging targets. Whether you need routine supply or specialized immunological reagents, CamelBio is your reliable development partner.