Anti-IL12B Rabbit Polyclonal Antibody for WB, ELISA - P29460

Core Product Specifications and Parameters

Biological Background: IL12B Function and Localization

• IL12B encodes the p40 subunit of interleukin-12 (IL-12), a heterodimeric pro-inflammatory cytokine central to cell-mediated immunity.
• Acts as a growth factor for activated T cells and NK cells, enhancing lytic activity and stimulating IFN-gamma production by PBMCs.
• Associates with IL23A to form interleukin-23 (IL-23), which activates Jak-Stat signaling and promotes Th17 responses, contributing to autoimmune inflammation.
• Secreted protein containing an immunoglobulin-like domain; undergoes glycosylation and disulfide bond formation.
• Bridges innate and adaptive immunity; IL-12 drives Th1 responses, while IL-23 drives Th17 responses, implicated in host defense and inflammatory disorders.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 37 kDa under reducing conditions; use cell lysates or supernatants from stimulated immune cells as positive controls.
• This polyclonal antibody recognizes multiple epitopes on the p40 subunit; it may detect both free p40 and p40-containing heterodimers (IL-12 and IL-23). Validate specificity in the relevant sample system.
• In ELISA, consider using this antibody as a detection reagent paired with a monoclonal capture antibody targeting a non-overlapping epitope.
• Due to cross-reactivity with mouse IL12B, it can be employed in mouse model studies; however, confirm reactivity in the specific model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. The IL12B Rabbit pAb targets a key immunoregulatory cytokine, making it valuable for immunology and inflammation research. We offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and rare target raw-material procurement to streamline your assay development.