Anti-IL12B Rabbit Monoclonal Antibody for WB, IHC-P, FC, ELISA - P43432

Core Product Specifications and Parameters

Biological Background: IL12B Function and Localization

• IL12B, also known as p40, is the beta subunit of interleukin-12 (IL-12) and interleukin-23 (IL-23), sharing a common subunit with distinct alpha subunits (IL12A and IL23A).
• As part of IL-12, it functions as a growth factor for activated T cells and natural killer (NK) cells, enhancing their lytic activity and stimulating IFN-gamma production by resting peripheral blood mononuclear cells (PBMCs).
• In the heterodimeric cytokine IL-23, it associates with IL23A to mediate innate and adaptive immunity, potentially contributing to acute responses to infection in peripheral tissues and promoting pro-inflammatory cytokine production.
• IL-23 signals through the IL12RB1/IL23R receptor complex, activating the JAK-STAT pathway, and primarily stimulates memory T cells, which may have implications in autoimmune inflammation and tumorigenesis.
• Subcellularly, IL12B is secreted and contains a signal peptide, an immunoglobulin domain, and undergoes post-translational modifications including glycosylation and disulfide bond formation.
• Keywords associated with IL12B include cytokine, glycoprotein, immunoglobulin domain, and disulfide bond, reflecting its structural and functional characteristics.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band around 38 kDa; verify with appropriate positive and negative controls.
• In immunohistochemistry (IHC-P), consider antigen retrieval conditions such as heat-induced epitope retrieval for optimal detection.
• For intracellular flow cytometry (FC), ensure proper fixation and permeabilization protocols are used.
• For ELISA, the antibody can be explored as a capture or detection reagent following pairing optimization.
• Given the immunogen spans the mature region, the antibody may recognize both the monomeric p40 and the heterodimer in native conditions; validate specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials, technical services, and consulting. For studies on interleukin signaling, immune regulation, or inflammatory diseases, we supply validated antibody pairs, optimized monoclonal antibodies like anti-IL12B, and bulk ancillary reagents to streamline your assay development. Our sourcing network can also assist with rare targets and custom raw-material procurement to bridge gaps in your workflow.