Anti-IL12A Polyclonal Antibody for WB, ELISA - P29459

Core Product Specifications and Parameters

Biological Background: IL12A Function and Localization

• IL12A (Interleukin-12 subunit alpha, also known as p35, CLMF, NFSK, NKSF1) is a cytokine subunit that forms the bioactive interleukin-12 (IL-12) by heterodimerizing with IL12B (p40) and interleukin-35 (IL-35) by associating with EBI3/IL27B. Related references: PMID:8605935, PMID:8943050
• IL-12 is predominantly secreted by antigen-presenting cells (B-cells, dendritic cells, macrophages, granulocytes) and critically regulates T-cell and natural killer (NK) cell responses, induces interferon-gamma (IFN-γ) production, and promotes T-helper 1 (Th1) cell differentiation, bridging innate and adaptive immunity. Related references: PMID:1673147, PMID:1674604, PMID:8605935
• The IL-12 receptor is composed of IL12R1 and IL12R2 subunits; ligand binding triggers tyrosine phosphorylation and activation of JAK family kinases TYK2 and JAK2, which then phosphorylate STAT4, leading to its nuclear translocation and regulation of responsive genes. Related references: PMID:8943050, PMID:7528775, PMID:7638186
• As part of IL-35, IL12A mediates immune-suppressive functions essential for maintaining liver immune homeostasis, signaling through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers, and uniquely requiring STAT1–STAT4 heterodimers for downstream signaling. Related references: PMID:22306691
• IL12A is secreted and undergoes co- and post-translational modifications including glycosylation and disulfide bond formation, which are important for proper folding and stability of the IL-12 heterodimer.
• The protein is highly conserved across human, mouse, and rat, making it a suitable target for cross-species studies.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was generated against a synthetic peptide spanning amino acids 50–150 of human IL12A (NP_000873.2). It is expected to recognize both the monomeric IL12A subunit and, under non-reducing conditions, possibly the IL-12 p70 heterodimer.
• For Western blotting, a band corresponding to the 25 kDa IL12A protein is anticipated in lysates from human, mouse, and rat tissues or cell lines (e.g., activated dendritic cells, macrophages). Optimize sample preparation to retain the secreted form; include appropriate controls.
• In ELISA applications, the antibody may be used for capture or detection of native or recombinant IL-12, but careful titration and pairing with a compatible detection antibody are recommended.
• Due to its polyclonal nature, batch-to-batch consistency should be validated in the relevant experimental system prior to large-scale studies.

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