Anti-IL11RA Rabbit mAb for WB - Q14626

Core Product Specifications and Parameters

Biological Background: IL11RA Function and Localization

• Interleukin-11 receptor subunit alpha (IL11RA), also known as CRSDA, is a transmembrane receptor that also exists as a soluble form (sIL11RA) generated by alternative splicing.

• IL11RA specifically binds interleukin-11 (IL11) and, together with the common signal transducer IL6ST/gp130, mediates signaling pathways that control proliferation and differentiation of skeletogenic progenitors and other mesenchymal cells.

• The soluble form sIL11RA acts as an agonist of IL11 activity, enabling IL11 trans-signaling on cells that lack membrane-bound IL11RA by forming an IL11:sIL11RA complex that engages IL6ST/gp130 on the cell surface. Related references: PMID:26876177 PMID:30279168

• IL11RA is essential for normal craniofacial bone and tooth development; it restricts suture fusion and regulates tooth number.

• The receptor is expressed in various cell lines, including myelogenous leukemia K-562, megakaryocytic leukemia M-07e, erythroleukemia TF-1, and osteosarcoma MG-63 and SaOS-2. Expression is also detected in normal and malignant prostate epithelial cells, with elevated levels in prostate carcinoma. Related references: PMID:7670098

• Subcellularly, IL11RA localizes to the plasma membrane and is also secreted as the soluble form.

• Key structural features include an immunoglobulin-like domain, disulfide bonds, and N-glycosylation sites, classifying it as a glycoprotein receptor.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 323–422) lies in the extracellular region of human IL11RA; sequence conservation across species may account for the observed cross-reactivity with mouse and rat.
• For Western blot, the predicted molecular weight is approximately 45 kDa; however, glycosylation can lead to a higher apparent mass. Consider using a suitable loading control and validate band specificity using knockdown or knockout samples when available.
• Standard antigen retrieval methods (e.g., heat-induced citrate buffer) are recommended for IHC-P. Include isotype controls and verify staining patterns against known tissue expression data.
• For IF/ICC, mild permeabilization may be required to access the intracellular epitope. Co-staining with organelle markers can help confirm membrane localization.
• Validate all applications in the relevant sample system and under optimized conditions; titration of the antibody is advisable for each new experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, labs, and research institutes with a comprehensive and streamlined sourcing solution for IVD raw materials, technical services, and expert consulting—guiding projects from concept to clinic. This IL11RA rabbit monoclonal antibody exemplifies our commitment to offering high-quality, validated reagents for immunoassays in cancer biology and bone development research. As your strategic partner, CamelBio supplies not only optimized monoclonal and polyclonal antibodies but also bulk ancillary reagents, validated antibody pairs, and tailored support for rare or custom targets to empower your assay development and manufacturing pipeline.