Anti-IL10 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P22301

Core Product Specifications and Parameters

Biological Background: IL10 Function and Localization

• Interleukin-10 (IL10), also known as cytokine synthesis inhibitory factor (CSIF), is a major anti-inflammatory cytokine that acts on various immune cells to limit excessive tissue disruption caused by inflammation.
• IL10 binds to the heterotetrameric receptor comprising IL10RA and IL10RB, leading to JAK1 and STAT2-mediated phosphorylation and activation of STAT3. Related references: PMID:16982608
• Activated STAT3 translocates to the nucleus to drive expression of anti-inflammatory mediators and inhibit production of pro-inflammatory cytokines such as GM-CSF, G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8, and TNF in antigen-presenting cells. Related references: PMID:18025162 PMID:11564774 PMID:1940799 PMID:7512027
• IL10 interferes with antigen presentation by reducing the expression of MHC class II and co-stimulatory molecules, thereby inhibiting T cell activation. Related references: PMID:8144879
• IL10 is a secreted cytokine produced by a variety of cell types including T-cells, macrophages, and mast cells.
• The protein contains characteristic features of a secreted glycoprotein, including a signal sequence, disulfide bonds, and glycosylation sites.
• IL10 also controls macrophage inflammatory responses by reprogramming essential metabolic pathways such as mTOR signaling.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 19–178 of human IL10, covering a large portion of the mature protein, which may enable detection of multiple isoforms or precursor forms.
• For Western blotting, expect a band near 21 kDa; however, glycosylation and other modifications may shift the apparent molecular weight. Validate using appropriate positive and negative controls.
• In immunohistochemistry, we recommend testing known IL10-expressing tissues (e.g., inflamed or immune-rich samples) and optimizing antigen retrieval conditions.
• For ELISA applications, this antibody can be evaluated as a capture or detection reagent, but optimal pairing conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and optimized monoclonal and polyclonal antibodies. For immunology and inflammation research, our products—such as this IL10 Rabbit pAb—support the development of reliable immunoassays. In addition, we provide customized sourcing for rare-target reagents to help bridge the gap from concept to clinical application.