Anti-IL-6 Rabbit Polyclonal Antibody for WB, ELISA - P05231

Core Product Specifications and Parameters

Biological Background: IL-6 Function and Localization

• Interleukin-6 (IL-6) is a multifunctional cytokine that exerts its effects through binding to IL6R and gp130, triggering classic, trans-, and cluster signaling pathways (Probable).
• It is a potent inducer of the acute phase response, rapidly synthesized by myeloid cells in response to infection or tissue injury to aid host defense (Probable).
• In adaptive immunity, IL-6 is essential for B cell differentiation into immunoglobulin-secreting cells and drives CD4+ T cell differentiation toward Tfh and Th17 lineages (By similarity).
• Through trans-signaling and synergy with IL1B and TNF, IL-6 induces VEGF production, enhancing angiogenesis and vascular permeability. Related references: PMID:12794819 PMID:17075861
• Released as a myokine after muscle contraction, IL-6 promotes lipolysis and improves insulin resistance. Related references: PMID:20823453
• IL-6 plays a protective role in liver regeneration and controls iron metabolism by regulating hepcidin expression during inflammation. Related references: PMID:15124018
• The protein is secreted and produced by skeletal muscle, with structural features including disulfide bonds and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30-212 of human IL-6, a region conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blot, the predicted molecular weight is approximately 24 kDa; however, due to glycosylation, it may appear at higher molecular weights. Validate in the relevant sample system.
• In ELISA, the antibody can be considered for capture or detection roles; optimization of coating and detection conditions is recommended.
• As IL-6 is a secreted protein, appropriate sample preparation (e.g., serum, plasma, or cell culture supernatant) is essential for accurate detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD raw materials, including this IL-6 polyclonal antibody for inflammation and immune response studies. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your development from concept to clinic.