Anti-IL-6 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, ELISA - P20607

Core Product Specifications and Parameters

Biological Background: Interleukin-6 Function and Localization

• Interleukin-6 (IL-6) is a pleiotropic cytokine encoded by the Il6 gene in rats, also known as Ilg6 or Ifnb2. It belongs to the IL-6 family and is a key mediator of the acute phase response.
• IL-6 signals through a receptor complex involving IL6R and the common signaling subunit IL6ST/gp130, triggering pathways such as JAK/STAT. It can activate classic signaling, trans-signaling, or cluster signaling depending on receptor presentation.
• It plays a crucial role in innate and adaptive immunity: synthesized by myeloid cells upon TLR activation, promotes B-cell differentiation into antibody-secreting cells, and directs CD4+ T cell differentiation into Tfh and Th17 lineages.
• IL-6 is essential for bone homeostasis, angiogenesis, and metabolic regulation, including insulin sensitivity and glucose homeostasis via trans-signaling in the CNS.
• Additionally, IL-6 functions as a myokine released after muscle contraction and contributes to liver regeneration and iron metabolism by regulating hepcidin expression.
• The protein is secreted and contains a signal peptide, with post-translational modifications including phosphorylation and disulfide bond formation critical for its structure and function.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 24 kDa in rat or mouse tissue lysates; validate the antibody in your sample system with appropriate controls.
• In immunoprecipitation, the monoclonal nature may enhance specificity; titrate antibody and use appropriate lysis buffers to maintain protein complexes.
• For immunofluorescence/ICC, since IL-6 is secreted, consider using a protein transport inhibitor (e.g., brefeldin A) to retain intracellular pools, and co-stain with a Golgi marker.
• ELISA development using this antibody as a capture or detection reagent requires careful pairing validation; evaluate performance with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this IL-6 monoclonal antibody and complementary reagents ideal for developing immunoassays targeting inflammatory and immune markers. We leverage our supply network to provide validated antibody pairs, bulk ancillary reagents, and access to rare-target raw materials—ensuring streamlined procurement from concept to clinic.