Anti-IL-6 Rabbit Monoclonal Antibody for IHC-P, IF/ICC, ELISA - P20607

Core Product Specifications and Parameters

Biological Background: IL-6 Function and Localization

• IL-6 (Interleukin-6), also known as ILg6, Ifnb2, and IL6, is a ~24 kDa secreted cytokine encoded by the Il6 gene in rat.
• Binds to IL6R, then associates with IL6ST/gp130 to trigger intracellular signaling via classic, trans, or cluster signaling pathways.
• Potent inducer of the acute phase response; rapidly produced by myeloid cells upon pathogen recognition via TLRs, contributing to host defense.
• In adaptive immunity, drives B-cell differentiation into immunoglobulin-secreting cells and is essential for T follicular helper (Tfh) cell development and germinal center formation.
• Required for naive CD4+ T cell differentiation into the Th17 lineage; together with IL21, controls the early generation of Tfh cells critical for antibody responses to viral infection.
• Acts as a myokine, enhancing lipolysis and insulin resistance, and regulates energy/glucose homeostasis via GLP-1.
• Plays a protective role in liver injury and tissue regeneration; regulates iron metabolism by inducing hepcidin expression during inflammation.
• Subcellular location: Secreted. Keywords include acute phase, cytokine, disulfide bond, growth factor, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen spans the mature secreted form (residues 25–211), including receptor-binding regions; the antibody likely recognizes conformational epitopes important for functional studies.
• For IHC-P and IF/ICC, consider antigen retrieval methods (e.g., heat-induced epitope retrieval) and validate with positive/negative controls, given IL-6’s secreted nature and rapid induction.
• For ELISA, this monoclonal antibody can be used as a capture or detection antibody; pairing with a polyclonal antibody may improve sensitivity. Validate in relevant biological matrices (serum, plasma, cell culture supernatant).
• Timely sample collection after stimulation is critical to capture transient IL-6 expression peaks in experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-IL-6 rabbit monoclonal antibody supports advancements in immunology, inflammation, and metabolism research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your diagnostic development pipeline.