Anti-IL-33R/ST2 Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P14719

Core Product Specifications and Parameters

Biological Background: IL-33R/ST2 Function and Localization

• Interleukin-1 receptor-like 1 (IL-33R/ST2), also known as Ly84, ST2, T1, is encoded by the Il1rl1 gene in mouse and is a member of the interleukin-1 receptor family.
• Acts as a receptor for interleukin-33 (IL-33), playing critical roles in innate and adaptive immunity, tissue homeostasis, and responses to environmental stresses together with coreceptor IL1RAP. Related references: PMID:17675517 PMID:18450470 PMID:22660580
• Upon IL-33 binding, recruits MYD88, IRAK1, IRAK4, and TRAF6, leading to phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8.
• In alternatively activated macrophages, induces UCP2-dependent mitochondrial rewiring that attenuates reactive oxygen species generation and preserves the Krebs cycle for persistent itaconate production and GATA3-dependent differentiation. Related references: PMID:34644537
• Functions as an inhibitory receptor for IL-33 signaling through a splice variant.
• Subcellular localization: cell membrane (transmembrane receptor) and also secreted as a soluble form.
• Predominantly expressed in hematopoietic tissues, macrophage, erythroid, epithelial, and fibroblast cell lines; isoform A in brain astrocytes and microglia, isoform B in brain endothelial cells.
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, and ubiquitination; subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 27-332 of the extracellular domain, enabling recognition of both membrane-bound and soluble IL-33R/ST2 forms.
• For Western blot (WB), a band around 65 kDa is expected; note that glycosylation may cause a higher apparent molecular weight. Validate detection in mouse hematopoietic or macrophage cell lines (e.g., RAW 264.7).
• For immunofluorescence/immunocytochemistry (IF/ICC), confirm membrane and/or intracellular staining patterns in fixed/permeabilized cells.
• For flow cytometry (FC), use live, non-permeabilized cells to detect surface IL-33R/ST2, with appropriate isotype controls.
• For ELISA, consider pairing with a detection antibody recognizing a different epitope for sandwich assays.
• Always optimize experimental conditions (antibody dilution, incubation time, blocking) for your specific application and sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, offering validated monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents to support immunodiagnostic development. This anti-IL-33R/ST2 antibody, targeting a key immune and inflammation marker, exemplifies our commitment to connecting researchers and manufacturers with high-quality reagents for mouse model studies. From single clones to custom production, we streamline procurement from concept to clinic.