Anti-IL-1RAcP Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NPH3

Core Product Specifications and Parameters

Biological Background: IL-1RAcP Function and Localization

• IL-1RAcP (Interleukin-1 receptor accessory protein, also known as IL1R3 or C3orf13) is a coreceptor for IL-1 receptor family members, participating in IL-1, IL-33, and IL-36 signaling pathways.
• Associates with IL1R1 bound to IL1B to form the high-affinity interleukin-1 receptor complex, triggering intracellular signaling via TIR domain-mediated recruitment of TOLLIP, MYD88, and IRAK1/2, leading to NF-kB and MAPK pathway activation.
• Does not bind IL-1 independently; IL1RN (IL-1 receptor antagonist) prevents IL1R1-IL1RAcP complex formation, and the non-signaling IL1R2 decoy receptor can modulate cellular responses.
• Isoform 4 (soluble) lacks the transmembrane domain and cannot mediate canonical IL-1 signaling (PubMed:19481478); instead, it associates with secreted IL1R2 to enhance neutralization of IL1B (PubMed:12530978). Related references: PMID:19481478 PMID:12530978
• Bidirectionally involved in neuronal synapse differentiation via trans-synaptic binding to PTPRD, and may regulate NMDA receptor function by facilitating IL1B-induced Src phosphorylation.
• Subcellular localization includes cell membrane (full-length form) and secreted (soluble isoforms); detected in liver, skin, placenta, thymus, lung, and overexpressed in certain leukemias and inflammatory diseases.
• Undergoes post-translational modifications including disulfide bonds, glycosylation, and phosphorylation; the molecular weight of the unprocessed precursor is ~65.4 kDa.
• Tissue specificity: isoform 4 predominantly expressed in brain; overexpressed in chronic myeloid leukemia stem cells and acute myeloid leukemia; expressed in Th1 and Th2 cells.

Experimental Guidance and Technical Tips

• Given the polyclonal nature, this antibody may recognize multiple IL1RAP isoforms. Confirm detection of the specific isoform of interest in your experimental system.
• The immunogen spans the extracellular domain (aa 21-356), so the antibody is expected to detect both full-length membrane-bound and soluble secreted forms.
• For Western blotting, a band at ~65 kDa (unmodified) is expected; however, glycosylation may increase the apparent molecular weight to 70-80 kDa. Consider deglycosylation enzymes for precise size confirmation.
• In IHC-P, perform antigen retrieval and optimize primary antibody dilution; validate in known positive tissues such as liver, skin, or placenta.
• For ELISA, this antibody may serve as a capture or detection reagent; assess cross-reactivity with other IL-1 receptor family members if used in multiplex assays.
• Cross-reactivity is predicted with mouse and rat IL1RAP based on sequence homology, but confirm reactivity if moving to these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this validated anti-IL-1RAcP rabbit polyclonal antibody alongside a comprehensive portfolio of IVD raw materials, including optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. As your one-stop partner from concept to clinic, we support diagnostic manufacturers and researchers in inflammation, oncology, and neuroscience with reliable, high-quality reagents.