Anti-IL-1RAcP Monoclonal Antibody for WB, ELISA - Q9NPH3

Core Product Specifications and Parameters

Biological Background: IL-1RAcP Function and Localization

• Interleukin-1 receptor accessory protein (IL-1RAcP), also known as IL1R3 or C3orf13, is encoded by the IL1RAP gene and belongs to the interleukin-1 receptor family; it functions as a coreceptor for IL1R1, IL1RL2, and IL1RL1 in the IL-1, IL-36, and IL-33 signaling systems, respectively.
• As a coreceptor with IL1R1, IL-1RAcP forms the high-affinity interleukin-1 receptor complex upon IL-1B binding, initiating signaling cascades that involve the recruitment of adapter molecules TOLLIP, MYD88, and IRAK1/2, leading to NF-kappa-B activation.
• It does not bind interleukin-1 independently; the antagonist IL1RN prevents its association with IL1R1, and the cellular response is further modulated through non-signaling interactions with the membrane-bound decoy receptor IL1R2.
• Secreted IL-1RAcP associates with soluble IL1R2 to potently neutralize IL-1B, representing a key regulatory mechanism. Related references: PMID:12530978
• Although IL-1RAcP is unable to mediate canonical IL-1 signaling on its own, it is required for Src phosphorylation by IL1B and may facilitate IL-1B-potentiated NMDA-induced calcium influx in neurons. Related references: PMID:19481478
• Multiple isoforms exist due to alternative splicing; isoform 4 is predominantly expressed in brain, while other isoforms are detected in liver, skin, placenta, thymus, and lung, with overexpression in chronic myeloid leukemia, acute myeloid leukemia, and chronic obstructive pulmonary disease. Related references: PMID:10653850
• The protein is a glycoprotein containing immunoglobulin-like domains and a TIR domain; it undergoes phosphorylation and exists in both membrane-bound and secreted forms.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant protein corresponding to amino acids 391–470 of human IL-1RAcP (NP_002173.1), an intracellular region near the C-terminus; it recognizes the major 65 kDa isoform in WB, but consider that glycosylation and alternative splicing may produce additional bands.
• For Western blot, use the predicted molecular weight of 65 kDa as a guide and include appropriate positive and negative controls (e.g., cell lines known to express IL-1RAcP, such as THP-1 or primary immune cells).
• For ELISA applications, the antibody can be used as a capture or detection reagent; optimize concentrations and validate in the relevant sample system.
• The cross-reactivity with mouse and rat enables comparative studies, but confirm reactivity in your model system as epitope conservation may vary.
• Since the antibody is a rabbit monoclonal, use an anti-rabbit secondary antibody for detection; avoid cross-species interference when using in multiplex assays.

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CamelBio simplifies procurement of critical IVD raw materials, connecting diagnostic developers with high-quality antibodies and reagents. For researchers targeting the IL-1 pathway, we offer validated monoclonal antibodies like anti-IL-1RAcP, along with tailored sourcing of ancillary reagents and bulk supplies to accelerate assay development. Our one-stop approach supports every stage from concept to clinical application.