Anti-IL-15RA Rabbit Monoclonal Antibody for IF/ICC, FC, ELISA - Q60819

Core Product Specifications and Parameters

Biological Background: IL-15RA Function and Localization

• Interleukin-15 receptor subunit alpha (IL-15RA), also known as CD215, is a transmembrane receptor featuring a sushi domain and acting as the high-affinity receptor for IL-15.
• Signals both in cis and trans, where IL-15RA from one subset of cells presents IL-15 to neighboring IL2RG-expressing cells. Related references: PMID:17947230
• Belongs to the interleukin receptor family and plays a key role in immune cell development, homeostasis, and activation.
• Localizes to the membrane and nucleus membrane, with secretion into the extracellular space also reported.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and phosphorylation; alternative splicing generates multiple isoforms.
• Widely expressed across tissues, with additional functions in neutrophils involving SYK kinase binding and IL-15-induced phagocytosis (by similarity).
• Predicted molecular weight of ~28 kDa (UniProt: 28061 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 33–205), suggesting the antibody recognizes the receptor on the cell surface, making it suitable for flow cytometry and live-cell IF/ICC.
• For IF/ICC, consider permeabilization to detect nuclear membrane localization; validate in mouse-derived cells or tissues.
• In flow cytometry, verify binding to mouse immune cell subsets known to express IL-15RA, using appropriate isotype controls.
• ELISA may detect soluble IL-15RA in mouse serum or culture supernatants; confirm cross-reactivity with natural protein.
• As protein weight is ~28 kDa, be aware that glycosylation and isoform variants may shift the observed band in western blotting (application not listed, but can be tested).
• Always validate the antibody in your specific experimental context, using relevant positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated one-stop provider of IVD raw materials, empowering diagnostic manufacturers and research laboratories with a seamless supply chain from concept to clinic. This anti-IL-15RA monoclonal antibody exemplifies our commitment to serving the immunology and immuno-oncology research communities. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to accelerate your assay development and production needs.